Mouse Anti-EREG Recombinant Antibody (clone 9E5) (CAT#: PABL-084)
Recombinant Mouse Antibody (9E5) is capable of binding to Epiregulin, expressed in Chinese Hamster Ovary cells (CHO).
Specific Inquiry
Figure 3 Immunofluorescent staining of antibodies in human colorectal carcinoma cells.
DLD-1 and HCT116 cells, representing two human colorectal carcinoma cell lines, were fixed with paraformaldehyde and treated with or without 0.2% Triton X-100 solution (localization to endomembranes was observed; white arrow).
Lee, Y. H., Iijima, M., Kado, Y., Mizohata, E., Inoue, T., Sugiyama, A.,... & Kodama, T. (2013). Construction and characterization of functional anti-epiregulin humanized monoclonal antibodies. Biochemical and biophysical research communications, 441(4), 1011-1017.
Figure 4 Flow cytometry analysis.
Antibodies were tested for their ability bind to the surface of each cell line (DLD-1, HCT116, and AGS). Green line: 0.1 lg/mL, blue line: 0.5 lg/mL, purple line: 1 lg/mL, red line: negative control (0 lg/mL).
Lee, Y. H., Iijima, M., Kado, Y., Mizohata, E., Inoue, T., Sugiyama, A.,... & Kodama, T. (2013). Construction and characterization of functional anti-epiregulin humanized monoclonal antibodies. Biochemical and biophysical research communications, 441(4), 1011-1017.
Figure 5 Single-cycle kinetic analysis of 9E5, HM0, and HM1 antibody binding to an amine-coupled CM5 chip on Biacore.
hEpiregulin-Fc was injected at concentrations of 1.5 lM, 300, 60, 12, and 2.4 nM, respectively, and analyzed in duplicate binding cycles over three differing capacity reaction surfaces.
Lee, Y. H., Iijima, M., Kado, Y., Mizohata, E., Inoue, T., Sugiyama, A.,... & Kodama, T. (2013). Construction and characterization of functional anti-epiregulin humanized monoclonal antibodies. Biochemical and biophysical research communications, 441(4), 1011-1017.
Specifications
- Immunogen
- Human epiregulin
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human EREG
- Species Reactivity
- Human
- Clone
- 9E5
- Applications
- ELISA, WB, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Immunofluorescence and Flow Cytometry. For details, refer to published data.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2396z | Mouse Anti-EREG Recombinant Antibody (clone 43A4) | WB, IHC | Mouse IgG1 |
| MOB-0693CT | Recombinant Mouse anti-Human EREG Monoclonal antibody (NN0376- 23Z5) | WB | |
| VS-0723-WK135 | Human Anti-EREG Recombinant Antibody (VS-0723-WK135) | FC | Chimeric (rabbit/human) IgG1 |
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABL-084, RRID: AB_3111626)
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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