Human Anti-FPR1 Recombinant Antibody (clone Fpro0165) (CAT#: PABL-097)

Figure 1 FACS staining was carried out using Fpro0165 IgG1 and control IgG1 with parental, human FPR2-overexpressing and human CXCR3-overexpressing HEK cells to determine the binding specificity of Fpor0165 to FPR1.

Douthwaite, J. A., Sridharan, S., Huntington, C., Hammersley, J., Marwood, R., Hakulinen, J. K.,... & Seaman, J. C. (2015, January). Affinity maturation of a novel antagonistic human monoclonal antibody with a long VH CDR3 targeting the Class A GPCR formyl-peptide receptor 1. In MAbs (Vol. 7, No. 1, pp. 152-166). Taylor & Francis.

Figure 2 Lack of inhibition of WKYMVM-induced calcium release in FPR2-overexpressing cells by Fpro0165 (B, right panel). Different agonist activity of the FPR1-specific formyl peptides fMLFF (EC50 not quoted, incomplete curve) and fMIFL (EC50, 0.12 nM on FPR1 cells) and FPR2-specific peptide WKYMVM (EC50, 0.49 nM on FPR2 cells) are shown in the left and center panels confirming the overexpression of FPR1 and FPR2 respectively.

Douthwaite, J. A., Sridharan, S., Huntington, C., Hammersley, J., Marwood, R., Hakulinen, J. K.,... & Seaman, J. C. (2015, January). Affinity maturation of a novel antagonistic human monoclonal antibody with a long VH CDR3 targeting the Class A GPCR formyl-peptide receptor 1. In MAbs (Vol. 7, No. 1, pp. 152-166). Taylor & Francis.

Figure 3 Determination of the apparent affinity of Fpro0165 for human FPR1 and cynomolgus FPR1 using Schild analysis of activity in a CD11b upregulation assay using in primary human and cynomolgus neutrophils.

Concentrationresponse curves of fMLFF induced CD11b expression in primary human (A) and cynomolgus (B) neutrophils in the absence (0) or presence of different concentrations Fpro0165 IgG (indicated in the key as logM). Schild plot analysis of the data derived from human (C) and cynomolgus (D) assays to generate the apparent Kd of the antibody. Dose ratios were generated using 50% response levels of fMLFF in the presence of antibody and log (dose ratio-1) was plotted against log [antibody] (M). The x-intercept of the fitted regression line is an estimate of pA2, the apparent equilibrium dissociation constant for the antibody.

Douthwaite, J. A., Sridharan, S., Huntington, C., Hammersley, J., Marwood, R., Hakulinen, J. K.,... & Seaman, J. C. (2015, January). Affinity maturation of a novel antagonistic human monoclonal antibody with a long VH CDR3 targeting the Class A GPCR formyl-peptide receptor 1. In MAbs (Vol. 7, No. 1, pp. 152-166). Taylor & Francis.