Mouse Anti-Fluorescein Recombinant Antibody (clone 43.1)
CAT#: PABL-096
Recombinant Mouse Antibody (43.1) is capable of binding to fluorescein's carboxyl phenyl moiety, expressed in Chinese Hamster Ovary cells (CHO). It demonstrates a very high affinity (KD ∼ 70 pM) and a fast association rate (kon ∼ 2 × 10(7) M(-1) s(-1)).
Published Data
Figure 2 Spectra and fluorescence decay of fluorescein, free and bound to mAb 43. Normalized absorption and emission spectra of free (A) and antibody bound (B) fluorescein. Excitation spectra fully trace absorption spectra in both cases. C: Fluorescence time decay of free and antibody bound fluorescein. To obtain comparable signal for the highly quenched bound form the sample concentration and excitation power were increased. D,E: residuals to the fit with three lifetime components.
Gayda, S., Longenecker, K. L., Judge, R. A., Swift, K. M., Manoj, S., Linthicum, D. S., & Tetin, S. Y. (2016). Three‐dimensional structure, binding, and spectroscopic characteristics of the monoclonal antibody 43.1 directed to the carboxyphenyl moiety of fluorescein. Biopolymers, 105(4), 234-243.
Figure 3 Equilibrium binding plot and thermodynamic parameters of the mAb 43.1 - fluorescein interaction. A: The fluorescein concentration was kept at 20 nM while concentration of the antibody varied when performing equilibrium binding tittrations. Arrhenius plots calculated for the association (B) and dissociation (C) binding reactions and van‘t Hoff plot (D) with the Ka values calculated from the kinetics data.
Gayda, S., Longenecker, K. L., Judge, R. A., Swift, K. M., Manoj, S., Linthicum, D. S., & Tetin, S. Y. (2016). Three‐dimensional structure, binding, and spectroscopic characteristics of the monoclonal antibody 43.1 directed to the carboxyphenyl moiety of fluorescein. Biopolymers, 105(4), 234-243.
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Clone
- 43.1
- Applications
- ELISA, WB, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Fluorescein
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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See other products for "Clone 43.1"
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See other products for "Fluorescein"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-3 | Human Anti-Fluorescein Recombinant Antibody (clone 4-4-20) | WB, FC | Human IgG |
| DrMAB-13 | Mouse Anti-Fluorescein Recombinant Antibody (clone 4-4-20) | ELISA, WB, FC | Mouse IgG |
| DrMAB-23 | Rat Anti-Fluorescein Recombinant Antibody (clone 4-4-20) | ELISA, WB, FC | Rat IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-6 | Human Anti-Fluorescein Recombinant Antibody (clone 4-4-20); Fab Fragment | WB, FC | Human Fab |
| PFBZ-046 | Human Anti-Fluorescein Recombinant Antibody (clone 4M5.3); Fab Fragment | FC | Human Fab |
| PFBW-046 | Mouse Anti-Fluorescein Recombinant Antibody (clone 9-40); Fab Fragment | WB, IF, FuncS | Mouse Fab |
| PFBC-047 | Human Anti-Fluorescein Recombinant Antibody (clone E2); Fab Fragment | WB, FuncS | Human Fab |
| FAMAB-0141CQ-F(E) | Mouse Anti-Fluorescein Recombinant Antibody (clone 18-2-3); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-096 | Mouse Anti-Fluorescein Recombinant Antibody (clone 43.1); scFv Fragment | ELISA, WB, FuncS | Mouse scFv |
| PSBZ-046 | Human Anti-Fluorescein Recombinant Antibody (clone 4M5.3); scFv Fragment | FC | Human scFv |
| PSBW-046 | Mouse Anti-Fluorescein Recombinant Antibody (clone 9-40); scFv Fragment | WB, IF, FuncS | Mouse scFv |
| PSBC-047 | Human Anti-Fluorescein Recombinant Antibody (clone E2); scFv Fragment | WB, FuncS | Human scFv |
| FAMAB-0141CQ-S(P) | Mouse Anti-Fluorescein Recombinant Antibody (clone 18-2-3); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-037 | Recombinant Mouse Anti-fluorescein Antibody (PABX-037) | WB, ELISA, FuncS, PK | IgG |
| PABX-037-F (E) | Recombinant Mouse Anti-fluorescein Antibody Fab Fragment (PABX-037-F (E)) | WB, ELISA, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-037-S (P) | Recombinant Mouse Anti-fluorescein Antibody scFv Fragment (PABX-037-S (P)) | WB, ELISA, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0141CQ | Mouse Anti-Fluorescein Recombinant Antibody (clone 18-2-3) | ELISA | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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