Mouse Anti-Fluorescein Recombinant Antibody (clone 43.1) (CAT#: PABL-096)
Recombinant Mouse Antibody (43.1) is capable of binding to fluorescein's carboxyl phenyl moiety, expressed in Chinese Hamster Ovary cells (CHO). It demonstrates a very high affinity (KD ∼ 70 pM) and a fast association rate (kon ∼ 2 × 10(7) M(-1) s(-1)).
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Figure 2 Spectra and fluorescence decay of fluorescein, free and bound to mAb 43. Normalized absorption and emission spectra of free (A) and antibody bound (B) fluorescein. Excitation spectra fully trace absorption spectra in both cases. C: Fluorescence time decay of free and antibody bound fluorescein. To obtain comparable signal for the highly quenched bound form the sample concentration and excitation power were increased. D,E: residuals to the fit with three lifetime components.
Gayda, S., Longenecker, K. L., Judge, R. A., Swift, K. M., Manoj, S., Linthicum, D. S., & Tetin, S. Y. (2016). Three‐dimensional structure, binding, and spectroscopic characteristics of the monoclonal antibody 43.1 directed to the carboxyphenyl moiety of fluorescein. Biopolymers, 105(4), 234-243.
Figure 3 Equilibrium binding plot and thermodynamic parameters of the mAb 43.1 - fluorescein interaction. A: The fluorescein concentration was kept at 20 nM while concentration of the antibody varied when performing equilibrium binding tittrations. Arrhenius plots calculated for the association (B) and dissociation (C) binding reactions and van‘t Hoff plot (D) with the Ka values calculated from the kinetics data.
Gayda, S., Longenecker, K. L., Judge, R. A., Swift, K. M., Manoj, S., Linthicum, D. S., & Tetin, S. Y. (2016). Three‐dimensional structure, binding, and spectroscopic characteristics of the monoclonal antibody 43.1 directed to the carboxyphenyl moiety of fluorescein. Biopolymers, 105(4), 234-243.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Clone
- 43.1
- Applications
- ELISA, WB, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Fluorescein
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-3 | Human Anti-Fluorescein Recombinant Antibody (clone 4-4-20) | WB, FC | Human IgG |
| PABZ-046 | Human Anti-Fluorescein Recombinant Antibody (clone 4M5.3) | FC | Human IgG |
| PABW-046 | Mouse Anti-Fluorescein Recombinant Antibody (clone 9-40) | WB, IF, FuncS | Mouse IgG |
| PABC-047 | Human Anti-Fluorescein Recombinant Antibody (clone E2) | WB, FuncS | Human IgG |
| PABL-475 | Mouse Anti-Fluorescein Recombinant Antibody (clone mAb 4-4-20) | Neut | Mouse IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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