Human Anti-Fluorescein Recombinant Antibody (clone 4M5.3)
CAT#: PABZ-046
Recombinant Human Antibody (4M5. 3) is capable of binding to Fluorescein, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-Fluorescein mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-Fluorescein mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Specifications
- Host Species
- Human
- Type
- Human IgG
- Clone
- 4M5.3
- Applications
- FC
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody 4M5.3 has been reported in application of Flow Cytometry. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
Flow Cytometry: The reported concentration of 4M5.3 for flow cytometry was 10 μg/mL.
Target
- Alternative Names
- Fluorescein
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Thorough Datasheet
The comprehensive datasheet that came with the antibody was really beneficial; it included all the characteristics you needed as well as usage advice.
Outstanding Flexibility
This antibody performed admirably in a variety of settings, such as flow cytometry and Western blot. Its adaptability is a major benefit.
Reliable Outcomes
Every time we've utilized this antibody, the findings have been dependable and consistent. It is now an essential component of our study.
Q&As
-
Exist any suggested protocols?
A: Manufacturers typically provide protocols for certain applications like western blotting, flow cytometry, and ELISA.
-
Is there a bulk price for this product?
A: Yes, bulk amounts are available upon request for industrial or large-scale experimentation purposes.
-
What sort of paperwork is included with the antibody?
A: The antibody comes with a datasheet, safety data sheet (SDS), and certificate of analysis (CoA).
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone 4M5.3"
- CAT
- Product Name
See other products for "Fluorescein"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-3 | Human Anti-Fluorescein Recombinant Antibody (clone 4-4-20) | WB, FC | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-6 | Human Anti-Fluorescein Recombinant Antibody (clone 4-4-20); Fab Fragment | WB, FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-13 | Mouse Anti-Fluorescein Recombinant Antibody (clone 4-4-20) | ELISA, WB, FC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-23 | Rat Anti-Fluorescein Recombinant Antibody (clone 4-4-20) | ELISA, WB, FC | Rat IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-096 | Mouse Anti-Fluorescein Recombinant Antibody (clone 43.1) | ELISA, WB, FuncS | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-096 | Mouse Anti-Fluorescein Recombinant Antibody (clone 43.1); scFv Fragment | ELISA, WB, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-096 | Mouse Anti-Fluorescein Recombinant Antibody (clone 43.1); Fab Fragment | ELISA, WB, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABW-046 | Mouse Anti-Fluorescein Recombinant Antibody (clone 9-40) | WB, IF, FuncS | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBZ-046 | Human Anti-Fluorescein Recombinant Antibody (clone 4M5.3); Fab Fragment | FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBW-046 | Mouse Anti-Fluorescein Recombinant Antibody (clone 9-40); Fab Fragment | WB, IF, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBC-047 | Human Anti-Fluorescein Recombinant Antibody (clone E2); Fab Fragment | WB, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBZ-046 | Human Anti-Fluorescein Recombinant Antibody (clone 4M5.3); scFv Fragment | FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBW-046 | Mouse Anti-Fluorescein Recombinant Antibody (clone 9-40); scFv Fragment | WB, IF, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBC-047 | Human Anti-Fluorescein Recombinant Antibody (clone E2); scFv Fragment | WB, FuncS | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-472 | Mouse Anti-Fluorescein Recombinant Antibody scFv Fragment (PSBL-472) | Neut | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-037 | Recombinant Mouse Anti-fluorescein Antibody (PABX-037) | WB, ELISA, FuncS, PK | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-037-S (P) | Recombinant Mouse Anti-fluorescein Antibody scFv Fragment (PABX-037-S (P)) | WB, ELISA, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-037-F (E) | Recombinant Mouse Anti-fluorescein Antibody Fab Fragment (PABX-037-F (E)) | WB, ELISA, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0141CQ | Mouse Anti-Fluorescein Recombinant Antibody (clone 18-2-3) | ELISA | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1124-YC430 | DrugMonitor™ Anti-Fluorescein Antibody (VS-1124-YC430) | ELISA, PK/PD, ADA control | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-YC71 | DrugMonitor™ Mouse Anti-Fluorescein Antibody (VS-0125-YC71) | ELISA, Lateral Flow | Mouse IgG |
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