Mouse Anti-GPRC5A Recombinant Antibody (clone 39A6)
CAT#: MOB-2559z
This product is a mouse antibody that recognizes human GPRC5A. It can be used in the following applications: FCM, ICC, IHC.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli, plasma membrane & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7928/76_B5_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46526/847_E5_2_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7928/155465_B_8_5.jpg
Normal Tissue
Figure 4 Lung
Alveolar cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Macrophages Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7928/155465_A_3_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7928/155465_A_7_5.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (A-431, A549, CAPAN-2, HaCaT, SiHa)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000013588-GPRC5A
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Specifications
- Immunogen
- Human G Protein-Coupled Receptor, Class C, Group 5, Member A
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG2a
- Antibody Isotype
- IgG2a
- Specificity
- Human GPRC5A
- Species Reactivity
- Human
- Clone
- 39A6
- Applications
- FC, ICC, IHC
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry.
Target
- Alternative Names
- GPRC5A; TPA Induced Gene 1; TIG1; G Protein-Coupled Receptor, Family C, Group 5, Member A; RAIG1; Retinoic Acid-Induced Gene 1 Protein; Phorbol Ester Induced Protein-1; G Protein-Coupled Receptor, Class C, Group 5, Member A; RAIG-1; G-Protein Coupled Rece
- UniProt ID
- Q8NFJ5
REVIEWS AND Q&AS
CITATIONS
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Verified For Multiple Apps
Clone 39A6 is a versatile mouse antibody. We have successfully used it in Flow Cytometry, Immunocytochemistry, and Immunohistochemistry to study Human GPRC5A expression. It consistently recognizes the target receptor, helping us investigate its role in epithelial cell differentiation.
Clear Staining Results
Our research involves the retinoic acid-inducible GPCR, GPRC5A. This recombinant antibody provided clear staining in our tissue samples. The specificity is excellent, and it has allowed us to gather evidence on the interaction between retinoid and G-protein signaling pathways.
Q&As
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What applications has this antibody been reported for?
A: This mouse antibody (Clone 39A6) has been reported for use in Flow Cytometry (FC), Immunocytochemistry (ICC), and Immunohistochemistry (IHC). It recognizes human GPRC5A, a retinoic acid-inducible GPCR.
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What is the isotype of this mouse antibody?
A: This product is a Mouse IgG2a isotype antibody. It is a recombinant antibody expressed in a mouse host and is designed to recognize the Human GPRC5A protein with high specificity.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# MOB-2559z, RRID: AB_3713973)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "GPRC5A"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2896 | Anti-GPRC5A Immunohistochemistry Kit | IHC |
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