Mouse Anti-GRPR Recombinant Antibody (clone 39B3)
CAT#: MOB-1749z
This product is a mouse antibody that recognizes human GRPR. It can be used in the following applications: WB, ELISA, IF, IP.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69604/1440_G3_4_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Group enriched (PC-3, T-47d, U-138 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000126010-GRPR
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Specifications
- Immunogen
- Human Gastrin-Releasing Peptide Receptor
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG2a, κ
- Antibody Isotype
- IgG2a, κ
- Specificity
- Human GRPR
- Species Reactivity
- Human
- Clone
- 39B3
- Applications
- WB, ELISA, IF, IP
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western blot, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Immunoprecipitation.
Target
- Alternative Names
- GRPR; GRP-Preferring Bombesin Receptor; BB2; Gastrin-Releasing Peptide Receptor; GRP-R
- UniProt ID
- P30550
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Specific GRPR Detection in IF
This mouse antibody (clone 39B3) worked very well for Immunofluorescence staining of GRPR in our cell culture models. We are studying the gastrin-releasing peptide receptor in the context of lung cancer, and this reagent allowed us to visualize receptor expression clearly. The IgG2a isotype controls matched perfectly, confirming specificity.
Reliable for IP and WB
We have used this antibody for both Immunoprecipitation and Western Blotting. It successfully pulled down the GRPR protein from lysates and detected it on the blot. It is a robust tool for studying G-protein coupled receptors, and its ability to recognize the human antigen has been vital for our research.
Q&As
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Can this antibody detect GRPR in Immunofluorescence assays?
A: Yes, this Mouse Anti-GRPR Recombinant Antibody (clone 39B3) has been reported for use in Immunofluorescence (IF) assays. It specifically recognizes Human GRPR (Gastrin-Releasing Peptide Receptor). It is also suitable for Western Blot (WB), Enzyme-linked Immunosorbent Assay (ELISA), and Immunoprecipitation (IP).
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What is the function of the protein targeted by this antibody?
A: The target, Gastrin-releasing peptide receptor (GRPR), regulates functions of the gastrointestinal and central nervous systems, including hormone release and smooth muscle contraction. It is a G-protein coupled receptor that activates the phospholipase C signaling pathway and is often aberrantly expressed in cancers of the lung, colon, and prostate.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
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COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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