Mouse Anti-HIV-1 gag-pol Recombinant Antibody (clone 25.3)
CAT#: PABC-059
Recombinant Mouse Antibody (25. 3) is capable of binding to HIV-1 gag-pol, expressed in mammalian cells.
Specifications
- Immunogen
- HIV-1 Gag-Pol
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Tested positive against HIV-1 gag-pol
- Species Reactivity
- HIV-1
- Clone
- 25.3
- Applications
- WB, IF, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Specific For Gag-Pol Polyprotein
We employed clone 25.3 for Western Blotting to detect HIV-1 Gag-Pol expression in our transfected cell lines. The antibody specifically bound to the target with high sensitivity. It is an essential reagent for our retroviral assembly studies, providing clear confirmation of protein production in mammalian cells.
Great For Immunofluorescence
In addition to Western blots, we used this recombinant mouse antibody for Immunofluorescence. It allowed us to visualize the intracellular distribution of the viral proteins. The lack of preservatives in the buffer was beneficial, as it minimized potential toxicity during our live-cell staining optimization steps.
Q&As
-
Is this a recombinant antibody, and what clone is it?
A: Yes, this is a Recombinant Mouse Antibody, Clone 25.3. It is capable of binding to HIV-1 gag-pol and is expressed in mammalian cells (CHO). Recombinant production allows for high consistency and scalability compared to traditional hybridomas.
-
What specific applications is this antibody recommended for?
A: This antibody is recommended for Western Blot (WB), Immunofluorescence (IF), and Functional Studies (FuncS). It has been tested positive against HIV-1 gag-pol. It is supplied in PBS without preservatives, so proper storage at -20°C is essential.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Clone 25.3"
- CAT
- Product Name
See other products for "HIV-1 gag-pol"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABC-060 | Mouse Anti-HIV-1 gag-pol peptide(GATPQDLNTnL, n=norleucine) Recombinant Antibody (clone CB41) | ELISA | Mouse IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBC-059 | Mouse Anti-HIV-1 gag-pol Recombinant Antibody (clone 25.3); Fab Fragment | WB, IF, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBC-060 | Mouse Anti-HIV-1 gag-pol peptide(GATPQDLNTnL, n=norleucine) Recombinant Antibody (clone CB41); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBC-059 | Mouse Anti-HIV-1 gag-pol Recombinant Antibody (clone 25.3); scFv Fragment | WB, IF, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBC-060 | Mouse Anti-HIV-1 gag-pol peptide(GATPQDLNTnL, n=norleucine) Recombinant Antibody (clone CB41); scFv Fragment | ELISA | Mouse scFv |
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