Recombinant Mouse Anti-HRV2 VP2 Antibody (8F5) (CAT#: PABL-201)

Figure 1 HeLa-FcRII cells were grown on coverslips, incubated with 8F5 (40 μg/ml) or with 3B10 (15 μg/ml) at 4°C for 1 h, washed, fixed, and stained with Alexa 488-conjugated goat anti-mouse antibodies. Subsequently, cells were incubated with 2.4G2 followed by Alexa 568-conjugated goat anti-rat antibody. Nuclei were stained with Hoechst dye. Note the absence of Alexa 488 staining in cells that do not express FcRII (arrows). Bar, 20 μm.

Baravalle, G., Brabec, M., Snyers, L., Blaas, D., & Fuchs, R. (2004). Human rhinovirus type 2-antibody complexes enter and infect cells via Fc-γ receptor IIB1. Journal of virology, 78(6), 2729-2737.

Figure 2 Parental HeLa cells or HeLa-FcRII cells were incubated with 8F5 (40 μg/ml) or 3B10 (15 μg/ml) at 4°C for 1 h, washed, fixed, and stained with Alexa 488-conjugated goat anti-mouse antibodies followed by incubation with 2.4G2 and Alexa 568-conjugated goat anti-rat antibodies. Cells were analyzed by flow cytometry (for details, see Materials and Methods). One typical experiment out of three is shown. Note that only those cells that express FcRII bound the MAbs.

Baravalle, G., Brabec, M., Snyers, L., Blaas, D., & Fuchs, R. (2004). Human rhinovirus type 2-antibody complexes enter and infect cells via Fc-γ receptor IIB1. Journal of virology, 78(6), 2729-2737.

Figure 3 MAbs 8F5 and 3B10 are internalized into HeLa-FcRII cells.

Cells were incubated at 34°C with 8F5 (40 μg/ml), 3B10 (15 μg/ml), or the control antibody 5H5 subtype IgG3 (40 μg/ml) for 60 min (8F5, 5H5) or for 20 min (3B10). Cells were washed, permeabilized (A) or nonpermeabilized (B), and processed for immunofluorescence microscopy as in Fig. ​Fig.2.2. Note the perinuclear colocalization of MAbs and FcRII in permeabilized cells, indicative of localization in late endosomes (arrowheads), and the absence of any antibody uptake in FcRII-negative cells (right-hand panels, arrows). The control antibody 5H5 was not internalized by HeLa-FcRII cells. Bar, 20 μm.

Baravalle, G., Brabec, M., Snyers, L., Blaas, D., & Fuchs, R. (2004). Human rhinovirus type 2-antibody complexes enter and infect cells via Fc-γ receptor IIB1. Journal of virology, 78(6), 2729-2737.