Recombinant Mouse Anti-HRV2 VP2 Antibody (8F5)
CAT#: PABL-201
Recombinant Mouse Neutralizing Antibody (8F5) is capable of binding to HRV2 VP2, expressed in Chinese Hamster Ovary cells (CHO).
Published Data
Figure 1 HeLa-FcRII cells were grown on coverslips, incubated with 8F5 (40 μg/ml) or with 3B10 (15 μg/ml) at 4°C for 1 h, washed, fixed, and stained with Alexa 488-conjugated goat anti-mouse antibodies. Subsequently, cells were incubated with 2.4G2 followed by Alexa 568-conjugated goat anti-rat antibody. Nuclei were stained with Hoechst dye. Note the absence of Alexa 488 staining in cells that do not express FcRII (arrows). Bar, 20 μm.
Baravalle, G., Brabec, M., Snyers, L., Blaas, D., & Fuchs, R. (2004). Human rhinovirus type 2-antibody complexes enter and infect cells via Fc-γ receptor IIB1. Journal of virology, 78(6), 2729-2737.
Figure 2 Parental HeLa cells or HeLa-FcRII cells were incubated with 8F5 (40 μg/ml) or 3B10 (15 μg/ml) at 4°C for 1 h, washed, fixed, and stained with Alexa 488-conjugated goat anti-mouse antibodies followed by incubation with 2.4G2 and Alexa 568-conjugated goat anti-rat antibodies. Cells were analyzed by flow cytometry (for details, see Materials and Methods). One typical experiment out of three is shown. Note that only those cells that express FcRII bound the MAbs.
Baravalle, G., Brabec, M., Snyers, L., Blaas, D., & Fuchs, R. (2004). Human rhinovirus type 2-antibody complexes enter and infect cells via Fc-γ receptor IIB1. Journal of virology, 78(6), 2729-2737.
Figure 3 MAbs 8F5 and 3B10 are internalized into HeLa-FcRII cells.
Cells were incubated at 34°C with 8F5 (40 μg/ml), 3B10 (15 μg/ml), or the control antibody 5H5 subtype IgG3 (40 μg/ml) for 60 min (8F5, 5H5) or for 20 min (3B10). Cells were washed, permeabilized (A) or nonpermeabilized (B), and processed for immunofluorescence microscopy as in Fig. Fig.2.2. Note the perinuclear colocalization of MAbs and FcRII in permeabilized cells, indicative of localization in late endosomes (arrowheads), and the absence of any antibody uptake in FcRII-negative cells (right-hand panels, arrows). The control antibody 5H5 was not internalized by HeLa-FcRII cells. Bar, 20 μm.
Baravalle, G., Brabec, M., Snyers, L., Blaas, D., & Fuchs, R. (2004). Human rhinovirus type 2-antibody complexes enter and infect cells via Fc-γ receptor IIB1. Journal of virology, 78(6), 2729-2737.
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Specifications
- Immunogen
- HRV2 Capsid protein VP2
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native HRV2 VP2
- Species Reactivity
- HRV2
- Clone
- 8F5
- Applications
- WB, ELISA, IF, FuncS, FC
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The antibody was validated for IF and FC. For details, refer to published data.
Target
- Alternative Names
- VP2; Capsid protein VP2; Rhinovirus a
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-201 | Recombinant Mouse Anti-HRV2 VP2 Antibody Fab Fragment (8F5) | WB, ELISA, IF, FuncS | Fab |
| FAMAB-0140-CN-F(E) | Recombinant Human Anti-VP2 Antibody Fab Fragment (860-55D) | WB, ELISA, FC | Human Fab |
| FAMAB-0188-CN-F(E) | Recombinant Chicken Anti-VP2 Antibody Fab Fragment (Fab-A) | WB, ELISA | Chicken Fab |
| FAMAB-0189-CN-F(E) | Recombinant Chicken Anti-VP2 Antibody Fab Fragment (Fab-B) | WB, ELISA | Chicken Fab |
| FAMAB-0190-CN-F(E) | Recombinant Chicken Anti-VP2 Antibody Fab Fragment (Fab-C) | WB, ELISA | Chicken Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0295YC | Recombinant Chicken Anti-VP2 Antibody (CRAb 88) | ELISA, IP, WB | Chicken IgG |
| FAMAB-0140-CN | Recombinant Human Anti-VP2 Antibody (860-55D) | WB, ELISA, FC, IP | Human IgG1, λ |
| FAMAB-0188-CN | Recombinant Chicken Anti-VP2 Antibody (scFv-A) | WB, ELISA | Chicken IgG |
| HPAB-0133-WJ | Recombinant Mouse Anti-VP2 Antibody (# 4) | ELISA | Mouse IgG2b |
| HPAB-0134-WJ | Recombinant Mouse Anti-VP2 Antibody (# 6) | ELISA | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0295-YC-S(P) | Recombinant Chicken Anti-VP2 Antibody scFv Fragment (CRAb 88) | ELISA, IP, WB | Chicken scFv |
| FAMAB-0140-CN-S(P) | Recombinant Human Anti-VP2 Antibody scFv Fragment (860-55D) | WB, ELISA, FC | Human scFv |
| FAMAB-0188-CN-S(P) | Recombinant Chicken Anti-VP2 Antibody scFv Fragment (scFv-A) | WB, ELISA | Chicken scFv |
| FAMAB-0189-CN-S(P) | Recombinant Chicken Anti-VP2 Antibody scFv Fragment (scFv-B) | WB, ELISA | Chicken scFv |
| FAMAB-0190-CN-S(P) | Recombinant Chicken Anti-VP2 Antibody scFv Fragment (scFv-C) | WB, ELISA | Chicken scFv |
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