Anti-Human HSP90 Recombinant Antibody (CAT#: TAB-368CL)

Figure 1 Anti-Human HSP90 Recombinant Antibody (TAB-368CL) in WB

Western blot analysis was performed by loading 0.1 µg (Lane 1), 0.3 µg(Lane 2), and 0.6 µg (Lane 3) recombinant human HSP90 onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least 1 hour. Membranes were probed with Anti-Human HSP90 Therapeutic Antibody (TAB-368CL) at a dilution of 1:500 overnight at 4°C on a rocking platform. Then probed with Goat anti-Mouse IgG HRP secondary antibody at a dilution of 1:2,000 for one hour. Chemiluminescent was detected.

Figure 2 Anti-Human HSP90 Recombinant Antibody (TAB-368CL) in Dot Blot

Dot Blot analysis of TAB-368CL was performed by coating with human HSP90 antigen.
TAB-368CL incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)

Figure 3 Anti-Human HSP90 Recombinant Antibody (TAB-368CL) in ELISA

ELISA analysis of TAB-368CL was performed by coating with recombinant human HSP90 antigen. Then blocked with BSA, and incubated with Anti-Human HSP90 Therapeutic Antibody (TAB-368CL). The HRP-conjugated Goat anti-Mouse IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.