Recombinant Mouse anti-Human CENPF Monoclonal antibody (25D20 /2E9) (CAT#: MOB-0463CT)
Recombinant Mouse monoclonal antibody to Human CENPF, expressed in Chinese Hamster Ovary cells(CHO).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/52382/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/52382/if_selected.jpg
Figure 2 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70134/149748_A_7_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70134/149748_A_7_8.jpg
Figure 3 Cerebral cortex
(Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70134/149748_B_8_5.jpg
(Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70134/149748_B_8_5.jpg
Figure 4 Testis
(Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70134/149748_A_6_6.jpg
(Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70134/149748_A_6_6.jpg
Figure 5 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000117724-CENPF
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000117724-CENPF
Specifications
- Immunogen
- Fusion protein, corresponding to amino acids 1759-2093 of Human Mitosin/CENPF.
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- 25D20 /2E9
- Applications
- Used for immunoassay techniques such as: Flow Cytometry; Immunocytochemistry/Immunofluorescence; Immunohistochemistry (Frozen sections); Immunohistochemistry paraffin embedded sections; Immunoprecipitation; Western Blot
- Conjugate
- Unconjugated
Product Property
- Purity
- Immunogen affinity purified
- Format
- Liquid
- Concentration
- 1.0 mg/ml
- Buffer
- Phosphate buffered saline
- Storage
- Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- CENPF; centromere protein F, 350/400kDa; CENF; hcp-1; PRO1779; centromere protein F; mitosin; AH antigen; kinetochore protein CENPF; CENP-F kinetochore protein; cell-cycle-dependent 350K nuclear protein; centromere protein F, 350/400ka (mitosin); centromere protein F, 350/400kDa (mitosin);
- Gene ID
- 1063
- UniProt ID
- P49454
- Protein Refseq
- NP_057427
- Function
- chromatin binding; dynein binding; protein C-terminus binding; protein binding; protein homodimerization activity; transcription factor binding;
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3248z | Mouse Anti-CENPF Recombinant Antibody (clone 37H11) | WB, ICC, IF, IHC, FC, IP | Mouse IgG2a |
| VS13-YC212 | Rabbit Anti-CENPF Recombinant Antibody (VS13-YC212) | IHC-P | Rabbit IgG |
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