Recombinant Mouse anti-Human CENPF Monoclonal antibody (25D20 /2E9)
CAT#: MOB-0463CT
Recombinant Mouse monoclonal antibody to Human CENPF, expressed in Chinese Hamster Ovary cells(CHO).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52382/if_selected.jpg
Normal Tissue
Figure 2 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70134/149748_A_7_8.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70134/149748_B_8_5.jpg
Normal Tissue
Figure 4 Testis
Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70134/149748_A_6_6.jpg
RNA Expression
Figure 5 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000117724-CENPF
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Specifications
- Immunogen
- Fusion protein, corresponding to amino acids 1759-2093 of Human Mitosin/CENPF.
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- 25D20 /2E9
- Applications
- Used for immunoassay techniques such as: Flow Cytometry; Immunocytochemistry/Immunofluorescence; Immunohistochemistry (Frozen sections); Immunohistochemistry paraffin embedded sections; Immunoprecipitation; Western Blot
- Conjugate
- Unconjugated
Product Property
- Purity
- Immunogen affinity purified
- Format
- Liquid
- Concentration
- 1.0 mg/ml
- Buffer
- Phosphate buffered saline
- Storage
- Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- CENPF; centromere protein F, 350/400kDa; CENF; hcp-1; PRO1779; centromere protein F; mitosin; AH antigen; kinetochore protein CENPF; CENP-F kinetochore protein; cell-cycle-dependent 350K nuclear protein; centromere protein F, 350/400ka (mitosin); centromere protein F, 350/400kDa (mitosin);
- Gene ID
- 1063
- UniProt ID
- P49454
- Protein Refseq
- NP_057427
- Function
- chromatin binding; dynein binding; protein C-terminus binding; protein binding; protein homodimerization activity; transcription factor binding;
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "CENPF"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3248z | Mouse Anti-CENPF Recombinant Antibody (clone 37H11) | WB, ICC, IF, IHC, FC, IP | Mouse IgG2a |
| VS13-YC212 | Rabbit Anti-CENPF Recombinant Antibody (VS13-YC212) | IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1374 | Anti-CENPF Immunohistochemistry Kit | IHC | |
| VS-0525-XY1375 | Anti-Mouse CENPF Immunohistochemistry Kit | IHC |
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