Recombinant Mouse anti-Human CENPF Monoclonal antibody (25D20 /2E9) (CAT#: MOB-0463CT)
Recombinant Mouse monoclonal antibody to Human CENPF, expressed in Chinese Hamster Ovary cells(CHO).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/52382/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/52382/if_selected.jpg
Figure 2 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70134/149748_A_7_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70134/149748_A_7_8.jpg
Figure 3 Cerebral cortex
(Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70134/149748_B_8_5.jpg
(Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70134/149748_B_8_5.jpg
Figure 4 Testis
(Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70134/149748_A_6_6.jpg
(Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70134/149748_A_6_6.jpg
Figure 5 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000117724-CENPF
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000117724-CENPF
Specifications
- Immunogen
- Fusion protein, corresponding to amino acids 1759-2093 of Human Mitosin/CENPF.
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- 25D20 /2E9
- Applications
- Used for immunoassay techniques such as: Flow Cytometry; Immunocytochemistry/Immunofluorescence; Immunohistochemistry (Frozen sections); Immunohistochemistry paraffin embedded sections; Immunoprecipitation; Western Blot
- Conjugate
- Unconjugated
Product Property
- Purity
- Immunogen affinity purified
- Format
- Liquid
- Concentration
- 1.0 mg/ml
- Buffer
- Phosphate buffered saline
- Storage
- Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- CENPF; centromere protein F, 350/400kDa; CENF; hcp-1; PRO1779; centromere protein F; mitosin; AH antigen; kinetochore protein CENPF; CENP-F kinetochore protein; cell-cycle-dependent 350K nuclear protein; centromere protein F, 350/400ka (mitosin); centromere protein F, 350/400kDa (mitosin);
- Gene ID
- 1063
- UniProt ID
- P49454
- Protein Refseq
- NP_057427
- Function
- chromatin binding; dynein binding; protein C-terminus binding; protein binding; protein homodimerization activity; transcription factor binding;
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3248z | Mouse Anti-CENPF Recombinant Antibody (clone 37H11) | WB, ICC, IF, IHC, FC, IP | Mouse IgG2a |
| VS13-YC212 | Rabbit Anti-CENPF Recombinant Antibody (VS13-YC212) | IHC-P | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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