Recombinant Mouse anti-Human NCBP2 Monoclonal antibody (3724D4b)
CAT#: MOB-1138CT
Recombinant Mouse monoclonal antibody to Human NCBP2, expressed in Chinese Hamster Ovary cells(CHO).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44850/590_C1_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/62483/1148_A12_1_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44850/103927_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44850/104087_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44850/104087_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44850/104087_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Peritubular cells Staining: Low Intensity: Moderate Quantity: <25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44850/104087_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44850/104087_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000114503-NCBP2
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Specifications
- Immunogen
- Recombinant fragment: RGDNEEQEKL LKKSCTLYVG NLSFYTTEEQ IYELFSKSGD IKKIIMGLDK MKKTACGFCF VEYYSRADAE NAMRYINGTR LDDRIIRTDW DAGFKEGRQY GRGRSGGQV, corresponding to internal sequence amino acids 26-134 of Human NCBP2
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- 3724D4b
- Applications
- Used for immunoassay techniques such as: Dot blot; Immunocytochemistry/Immunofluorescence; Western Blot
- Conjugate
- Unconjugated
Product Property
- Purity
- Protein G purified
- Format
- Liquid
- Concentration
- 0.1 mg/ml
- Buffer
- Preservative: 0.05% Sodium Azide Constituents: 1% BSA, PBS, pH 7.4
- Storage
- Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- NCBP2; nuclear cap binding protein subunit 2, 20kDa; CBC2; NIP1; CBP20; PIG55; nuclear cap-binding protein subunit 2; NCBP 20 kDa subunit; NCBP interacting protein 1; NCBP-interacting protein 1; 20 kDa nuclear cap-binding protein; cell proliferation-inducing gene 55 protein; nuclear cap binding protein subunit 2, 20kD;
- Gene ID
- 22916
- UniProt ID
- P52298
- Protein Refseq
- NP_001036005
- Function
- RNA 7-methylguanosine cap binding; NOT RNA binding; RNA cap binding; nucleotide binding; protein binding;
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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|---|---|---|---|
| VS-0325-XY1458 | Anti-NCBP2 Immunohistochemistry Kit | IHC |
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