Recombinant Mouse anti-Human PLD1 Monoclonal antibody (EML673)
CAT#: MOB-0172CT
Recombinant Mouse monoclonal antibody to Human PLD1, expressed in Chinese Hamster Ovary cells(CHO).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line GAMG
Immunofluorescent staining of human cell line GAMG shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/66938/2058_H7_4_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human gallbladder
Immunohistochemical staining of human gallbladder shows strong membranous and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4527/12161_A_5_4_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Nuclear
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42396/116392_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42396/116392_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42396/116392_A_8_4.jpg
Normal Tissue
Figure 6 Gallbladder
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42396/116392_A_6_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42396/116392_A_7_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42396/116392_A_6_6.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42396/116392_A_9_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (HUVEC TERT2, SK-MEL-30, TIME)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000075651-PLD1/subcellular
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Specifications
- Immunogen
- Recombinant fragment, corresponding to amino acids 965-1075 of Human Phospholipase D1
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- EML673
- Applications
- Used for immunoassay techniques such as: Western Blot
- Conjugate
- Unconjugated
Product Property
- Purity
- Protein G purified
- Format
- Liquid
- Concentration
- Batch dependent within range:0.37 -0.37 mg/ml
- Buffer
- Preservative: None PBS, pH 7.2
- Storage
- Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- PLD1; phospholipase D1, phosphatidylcholine-specific; phospholipase D1; choline phosphatase 1; phosphatidylcholine-hydrolyzing phospholipase D1;
- Gene ID
- 5337
- UniProt ID
- Q13393
- Protein Refseq
- NP_001123553
- Function
- NAPE-specific phospholipase D activity; hydrolase activity; phosphatidylinositol binding; phospholipase D activity;
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Related Signaling Pathways
Ras Signaling Pathway
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
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COA
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See other products for "PLD1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2744 | Hi-Affi™ Recombinant Rabbit Anti-PLD1 Monoclonal Antibody (DS2744AB) | WB, FC, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1670 | Anti-PLD1 Immunohistochemistry Kit | IHC |
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