Recombinant Mouse anti-Human PRPF8 Monoclonal antibody (3945D2b) (CAT#: MOB-1717CT)
Recombinant Mouse monoclonal antibody to Human PRPF8, expressed in Chinese Hamster Ovary cells(CHO).
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9941/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9941/if_selected.jpg
Figure 2 IHC staining of human lung
(Immunohistochemical staining of human lung shows strong nuclear positivity in alveolar cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9941/ihc_selected.jpg
(Immunohistochemical staining of human lung shows strong nuclear positivity in alveolar cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9941/ihc_selected.jpg
Figure 3 Cerebral cortex
(Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15457/35849_B_7_5.jpg
(Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15457/35849_B_7_5.jpg
Figure 4 Colon
(Glandular cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15457/35849_A_7_3.jpg
(Glandular cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15457/35849_A_7_3.jpg
Figure 5 Liver
(Hepatocytes
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15457/35849_A_8_4.jpg
(Hepatocytes
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15457/35849_A_8_4.jpg
Figure 6 Kidney
(Cells in glomeruli
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
Cells in tubules
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15457/35849_A_7_5.jpg
(Cells in glomeruli
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
Cells in tubules
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15457/35849_A_7_5.jpg
Figure 7 Testis
(Leydig cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15457/35849_A_4_6.jpg
(Leydig cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15457/35849_A_4_6.jpg
Figure 8 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Non-germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15457/35849_A_8_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Non-germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15457/35849_A_8_8.jpg
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000174231-PRPF8/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000174231-PRPF8/subcellular
Specifications
- Immunogen
- Recombinant fragment (Human)
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- 3945D2b
- Applications
- Used for immunoassay techniques such as: Dot blot; Western Blot
- Conjugate
- Unconjugated
Product Property
- Purity
- Protein G purified
- Format
- Liquid
- Concentration
- 0.1 mg/ml
- Buffer
- Preservative: 0.05% Sodium Azide Constituents: 1% BSA, PBS, pH 7.4
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- PRPF8; pre-mRNA processing factor 8; PRP8; RP13; HPRP8; PRPC8; SNRNP220; pre-mRNA-processing-splicing factor 8; p220; PRP8 homolog; splicing factor Prp8; apoptosis-regulated protein 1; apoptosis-regulated protein 2; 220 kDa U5 snRNP-specific protein; precursor mRNA processing protein; PRP8 pre-mRNA processing factor 8 homolog; U5 snRNP-specific protein (220 kD), ortholog of S. cerevisiae Prp8p;
- Gene ID
- 10594
- UniProt ID
- Q6P2Q9
- Protein Refseq
- NP_006436
- Function
- RNA binding; protein binding;
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1284-CN | Recombinant Rabbit Anti-PRPF8 Monoclonal Antibody (JG57-38) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-WK506 | Rabbit Anti-PRPF8 Recombinant Antibody (clone JG57-38) | WB, ELISA | Rabbit IgG |
| VS3-FY1857 | Rabbit Anti-PRPF8 Recombinant Antibody (clone R06-1I7) | WB, IHC-P | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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