Rabbit Anti-PRPF8 Recombinant Antibody (clone R06-1I7)
CAT#: VS3-FY1857
This PRPF8 recombinant antibody binds specifically to human PRPF8, a 274 kDa member of the immunodlobulin superfamily. The antibody is recommended for detection of PRPF8 protein by WB, IHC-P. This antbody has been tested by western blot analysis of PRPF8 in Jurkat, Raji lysates.
Tested Data
Gene Expression
WB
Figure 1 Anti-PRPF8 Antibody (VS3-FY1857) in WB.
The PRPF8 expression in Jurkat, Raji lysates were detected using the anti-PRPF8 by western blot assay.
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9941/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human lung
Immunohistochemical staining of human lung shows strong nuclear positivity in alveolar cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9941/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15457/35849_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15457/35849_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15457/35849_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
Cells in tubules
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15457/35849_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15457/35849_A_4_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Non-germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15457/35849_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000174231-PRPF8/subcellular
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Specifications
- Immunogen
- Synthetic peptide of human PRPF8
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PRPF8
- Species Reactivity
- Human
- Clone
- R06-1I7
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 274 kDa, while the observed molecular weight stands at 274 kDa.
- Related Disease
- Retinitis Pigmentosa 13 and Retinitis Pigmentosa
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- PRPF8; pre-mRNA processing factor 8; HPRP8; PRP8; PRPC8; RP13; SNRNP220
- Gene ID
- 10594
- UniProt ID
- Q6P2Q9
- Cellular Localization
- Nucleus, Spliceosome
- Post Translation Modifications
- Ubiquitination at Lys36, Lys43, Lys93, Lys218, Lys267, Lys278, Lys301, Lys428, Lys442, Lys452, Lys460, Lys480, Lys525, Lys609, Lys702, Lys705, Lys727, Lys746, Lys785, Lys932, Lys1020, Lys1158, Lys1210, Lys1222, Lys1300, Lys1344, Lys1392, Lys1434, Lys1443, Lys1449, Lys1463, Lys1732, Lys1735, Lys1792, Lys1801, Lys1859, Lys1866, Lys1901, Lys2034, Lys2049, Lys2070, and Lys2140 (NX_Q6P2Q9 [NX_Q6P2Q9-1])
- Protein Refseq
- XP_024306305.1; XP_054170697.1; XP_054185172.1; NP_006436.3
- Function
- Plays a role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes, both of the predominant U2-type spliceosome and the minor U12-type spliceosome. Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome. Functions as a scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PRPF8"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1717CT | Recombinant Mouse anti-Human PRPF8 Monoclonal antibody (3945D2b) | Dot, WB | |
| MRO-1284-CN | Recombinant Rabbit Anti-PRPF8 Monoclonal Antibody (JG57-38) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-WK506 | Rabbit Anti-PRPF8 Recombinant Antibody (clone JG57-38) | WB, ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5745 | Anti-PRPF8 Immunohistochemistry Kit | IHC |
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