Recombinant Mouse anti-Human SFPQ Monoclonal antibody (7E8)
CAT#: MOB-1879CT
Recombinant Mouse monoclonal antibody to Human SFPQ, expressed in Chinese Hamster Ovary cells(CHO).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47513/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54689/if_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47513/134266_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47513/134266_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47513/134266_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47513/134266_A_8_5.jpg
Normal Tissue
Figure 7 Urinary bladder
Urothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47513/134266_A_6_5.jpg
Normal Tissue
Figure 8 Testis
Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47513/134266_A_4_6.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47513/134266_A_7_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000116560-SFPQ
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Specifications
- Immunogen
- Recombinant Human SFPQ protein .
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- 7E8
- Applications
- Used for immunoassay techniques such as: Immunocytochemistry/Immunofluorescence; Immunohistochemistry paraffin embedded sections; Western Blot; sEnzyme-linked Immunosorbent Assay
- Conjugate
- Unconjugated
Product Property
- Purity
- Protein A purified
- Format
- Liquid
- Concentration
- 0.48 mg/ml
- Buffer
- pH: 7.20Constituent:99% PBS
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- SFPQ; splicing factor proline/glutamine-rich; PSF; POMP100; PPP1R140; splicing factor, proline- and glutamine-rich; hPOMp100; 100 kDa DNA-pairing protein; PTB-associated splicing factor; PTB-associated-splicing factor; DNA-binding p52/p100 complex, 100 kDa subunit; protein phosphatase 1, regulatory subunit 140; polypyrimidine tract binding protein associated; polypyrimidine tract-binding protein-associated splicing factor; polypyrimidine tract-binding protein-associated-splicing factor; splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated); splicing factor proline/glutamine rich (polypyrimidine tract-binding protein-associated);
- Gene ID
- 6421
- UniProt ID
- P23246
- Protein Refseq
- NP_005057
- Function
- DNA binding; RNA binding; nucleotide binding; protein binding;
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| MOB-2221z | Mouse Anti-SFPQ Recombinant Antibody (clone 22B2) | WB, ICC, IF, IHC, IP | Mouse IgG1 |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3242 | Hi-Affi™ Recombinant Rabbit Anti-SFPQ Monoclonal Antibody (DS3242AB) | WB, IHC-P, ICC, IF | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2051 | Anti-SFPQ Immunohistochemistry Kit | IHC | |
| VS-0525-XY6526 | Anti-Mouse SFPQ Immunohistochemistry Kit | IHC | |
| VS-0525-XY6525 | Anti-Human SFPQ Immunohistochemistry Kit | IHC |
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