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  • Recombinant Mouse anti-Human SMARCA2 Monoclonal antibody (EML2252)

Recombinant Mouse anti-Human SMARCA2 Monoclonal antibody (EML2252) (CAT#: MOB-1887CT)

Recombinant Mouse monoclonal antibody to Human SMARCA2, expressed in Chinese Hamster Ovary cells(CHO).


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Specific Inquiry
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  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/371_H2_1_selected.jpg
Figure 2 IHC staining of human cerebral cortex
Figure 2 IHC staining of human cerebral cortex
(Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/ihc_selected.jpg
Figure 3 IF staining of human cell line A-431
Figure 3 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/374_H2_1_red_green.jpg
Figure 4 IF staining of human cell line U-251 MG
Figure 4 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/372_H2_1_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Figure 5 Cerebral cortex
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/69188_B_7_5.jpg
Figure 6 Parathyroid gland
Figure 6 Parathyroid gland
(Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/69498_A_3_8.jpg
Figure 7 Colon
Figure 7 Colon
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/69498_A_9_3.jpg
Figure 8 Liver
Figure 8 Liver
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/69498_A_8_4.jpg
Figure 9 Kidney
Figure 9 Kidney
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/69498_A_7_5.jpg
Figure 10 Testis
Figure 10 Testis
(Pachytene spermatocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Round or early spermatids
Staining:High
Intensity: Strong
Quantity: 75%-25%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/69498_A_5_6.jpg
Figure 11 Lymph node
Figure 11 Lymph node
(Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/69498_A_8_8.jpg
RNA Expression
Figure 12 RNA cell line category: Cell line enriched (HDLM-2)
Figure 12 RNA cell line category: Cell line enriched (HDLM-2)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000080503-SMARCA2

Specifications

  • Immunogen
  • Recombinant fragment: KILLDPNSEE VSEKDAKQII ETAKQDVDDE YSMQYSARGS QSYYTVAHAI SERVEKQSAL LINGTLKHYQ LQGLEWM, corresponding to amino acids 659-736 of Human SMARCA2 / BRM
  • Host Species
  • Mouse
  • Species Reactivity
  • Human
  • Clone
  • EML2252
  • Applications
  • Used for immunoassay techniques such as: Western Blot
  • Conjugate
  • Unconjugated

Product Property

  • Purity
  • Protein G purified
  • Format
  • Liquid
  • Concentration
  • 1.0 mg/ml
  • Buffer
  • Preservative: None PBS, pH 7.2
  • Storage
  • Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Target

  • Alternative Names
  • SMARCA2; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2; BRM; SNF2; SWI2; hBRM; NCBRS; Sth1p; BAF190; SNF2L2; SNF2LA; hSNF2a; probable global transcription activator SNF2L2; BAF190B; SNF2-alpha; protein brahma homolog; SNF2/SWI2-like protein 2; BRG1-associated factor 190B; ATP-dependent helicase SMARCA2; sucrose nonfermenting 2-like protein 2; global transcription activator homologous sequence; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a2;
  • Function
  • ATP binding; DNA binding; DNA-dependent ATPase activity; RNA polymerase II transcription coactivator activity; helicase activity; hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides; nucleotide binding; protein binding; tran

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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