Recombinant Mouse anti-Human SMARCA2 Monoclonal antibody (EML2252)

Name: Recombinant Mouse anti-Human SMARCA2 Monoclonal antibody (EML2252)
SKU: MOB-1887CT
Rating: 5 (1 reviews)

CAT#: MOB-1887CT

Recombinant Mouse monoclonal antibody to Human SMARCA2, expressed in Chinese Hamster Ovary cells(CHO).

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/371_H2_1_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human cerebral cortex

Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/ihc_selected.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/374_H2_1_red_green.jpg

Subcellular Location and Protein Expression Figure 4 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/372_H2_1_red_green.jpg

Normal Tissue Figure 5 Cerebral cortex

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/69188_B_7_5.jpg

Normal Tissue Figure 6 Parathyroid gland

Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/69498_A_3_8.jpg

Normal Tissue Figure 7 Colon

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/69498_A_9_3.jpg

Normal Tissue Figure 8 Liver

Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/69498_A_8_4.jpg

Normal Tissue Figure 9 Kidney

Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/69498_A_7_5.jpg

Normal Tissue Figure 10 Testis

Pachytene spermatocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Round or early spermatids
Staining:High
Intensity: Strong
Quantity: 75%-25%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/69498_A_5_6.jpg

Normal Tissue Figure 11 Lymph node

Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/69498_A_8_8.jpg

RNA Expression Figure 12 RNA cell line category: Cell line enriched (HDLM-2)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000080503-SMARCA2

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Specifications

Immunogen
Recombinant fragment: KILLDPNSEE VSEKDAKQII ETAKQDVDDE YSMQYSARGS QSYYTVAHAI SERVEKQSAL LINGTLKHYQ LQGLEWM, corresponding to amino acids 659-736 of Human SMARCA2 / BRM

Host Species
Mouse

Species Reactivity
Human

Clone
EML2252

Applications
Used for immunoassay techniques such as: Western Blot

Conjugate
Unconjugated

Product Property

Purity
Protein G purified

Format
Liquid

Concentration
1.0 mg/ml

Buffer
Preservative: None PBS, pH 7.2

Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Target

Alternative Names
SMARCA2; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2; BRM; SNF2; SWI2; hBRM; NCBRS; Sth1p; BAF190; SNF2L2; SNF2LA; hSNF2a; probable global transcription activator SNF2L2; BAF190B; SNF2-alpha; protein brahma homolog; SNF2/SWI2-like protein 2; BRG1-associated factor 190B; ATP-dependent helicase SMARCA2; sucrose nonfermenting 2-like protein 2; global transcription activator homologous sequence; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a2;

Gene ID
6595

UniProt ID
P51531

Protein Refseq
NP_003061

Function
ATP binding; DNA binding; DNA-dependent ATPase activity; RNA polymerase II transcription coactivator activity; helicase activity; hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides; nucleotide binding; protein binding; tran

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - MOB-1887CT.pdf

MSDS

MSDS - MOB-1887CT.pdf

COA

Certificate of Analysis Lookup

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See other products for "SMARCA2"

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CAT	Product Name	Application	Type
DrMAB-545	Recombinant Human Anti-Human SMARCA2 Antibody, clone D502	ELISA, IP-MS	IgG
VS13-YC1080	Rabbit Anti-SMARCA2 Recombinant Antibody (VS13-YC1080)	WB, IHC-Fr, IHC-P, ICC, IF	Rabbit IgG
VS7-0425-WR885	Mouse Anti-SMARCA2 Recombinant Antibody (clone 7A5B2 )	WB, IHC	Mouse IgG1
VS7-0425-WR886	Mouse Anti-SMARCA2 Recombinant Antibody (clone 3A7B4 )	WB, IHC, ICC, FC	Mouse IgG1
VS7-0425-WR887	Mouse Anti-SMARCA2 Recombinant Antibody (clone 10F4C9 )	WB, IHC, ICC, FC	Mouse IgG2a

CAT	Product Name	Application	Type
MHC-LC4435	PE-B*07:02/Human SMARCA2 (RPSGPGPEL) MHC Tetramer	FCM
MHC-LC4436	PE-A*69:01/Human SMARCA2 (RPSGPGPEL) MHC Tetramer	FCM

CAT	Product Name	Application	Type
VS-0325-XY2112	Anti-SMARCA2 Immunohistochemistry Kit	IHC
VS-0525-XY6729	Anti-Human SMARCA2 Immunohistochemistry Kit	IHC

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