Rabbit Anti-SMARCA2 Recombinant Antibody (VS13-YC1080)
CAT#: VS13-YC1080
The Rabbit Anti-SMARCA2 Recombinant Antibody is specific for human SMARCA2. SMARCA2 encodes a SWI/SNF family helicase/ATPase, regulating gene transcription by altering chromatin structure, involved in chromosomal disorders. The anti-SMARCA2 antibody can be used for Western blot (1/500-1/1000), Immunohistochemistry (1/50-1/100), Immunofluorescence (1/50-1/200).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/371_H2_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/374_H2_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/372_H2_1_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/69188_B_7_5.jpg
Normal Tissue
Figure 6 Parathyroid gland
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/69498_A_3_8.jpg
Normal Tissue
Figure 7 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/69498_A_9_3.jpg
Normal Tissue
Figure 8 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/69498_A_8_4.jpg
Normal Tissue
Figure 9 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/69498_A_7_5.jpg
Normal Tissue
Figure 10 Testis
Pachytene spermatocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Round or early spermatids
Staining:High
Intensity: Strong
Quantity: 75%-25%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/69498_A_5_6.jpg
Normal Tissue
Figure 11 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/69498_A_8_8.jpg
RNA Expression
Figure 12 RNA cell line category: Cell line enriched (HDLM-2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000080503-SMARCA2
❮
❯
Specifications
- Immunogen
- Recombinant human SMARCA2.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human SMARCA2
- Species Reactivity
- Human
- Applications
- WB, IHC-Fr, IHC-P, ICC, IF
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4) containing 150 mM NaCl, 40% Glycerol and 0.05% BSA.
- Preservative
- 0.01% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunohistochemistry (IHC): 1/50-1/100
Immunofluorescence (IF): 1/50-1/200
Target
- Alternative Names
- SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily A, Member 2; ATP-Dependent Helicase SMARCA2; BRG1-Associated Factor 190B; Protein Brahma Homolog; Brahma Homolog; SNF2-Alpha; BAF190B; SNF2L2; HBRM; BRM; SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily A Member 2; SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin A2; Global Transcription Activator Homologous Sequence; Probable Global Transcription Activator SNF2L2;
- Gene ID
- 6595
- UniProt ID
- P51531
- Long Name
- SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily A, Member 2
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "SMARCA2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-545 | Recombinant Human Anti-Human SMARCA2 Antibody, clone D502 | ELISA, IP-MS | IgG |
| MOB-1887CT | Recombinant Mouse anti-Human SMARCA2 Monoclonal antibody (EML2252) | WB | |
| VS7-0425-WR885 | Mouse Anti-SMARCA2 Recombinant Antibody (clone 7A5B2 ) | WB, IHC | Mouse IgG1 |
| VS7-0425-WR886 | Mouse Anti-SMARCA2 Recombinant Antibody (clone 3A7B4 ) | WB, IHC, ICC, FC | Mouse IgG1 |
| VS7-0425-WR887 | Mouse Anti-SMARCA2 Recombinant Antibody (clone 10F4C9 ) | WB, IHC, ICC, FC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC4435 | PE-B*07:02/Human SMARCA2 (RPSGPGPEL) MHC Tetramer | FCM | |
| MHC-LC4436 | PE-A*69:01/Human SMARCA2 (RPSGPGPEL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2112 | Anti-SMARCA2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6729 | Anti-Human SMARCA2 Immunohistochemistry Kit | IHC |
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