Recombinant Mouse anti-Human ZNF174 Monoclonal antibody (EML2238) (CAT#: MOB-2085CT)
Recombinant Mouse monoclonal antibody to Human ZNF174, expressed in Chinese Hamster Ovary cells(CHO).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line REH
(Immunofluorescent staining of human cell line REH shows localization to nuclear bodies & nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70692/2055_E10_2_selected.jpg
(Immunofluorescent staining of human cell line REH shows localization to nuclear bodies & nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70692/2055_E10_2_selected.jpg
Figure 2 IHC staining of human gallbladder
(Immunohistochemical staining of human gallbladder shows moderate nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9656/24924_A_5_4_selected.jpg
(Immunohistochemical staining of human gallbladder shows moderate nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9656/24924_A_5_4_selected.jpg
Figure 3 IF staining of human cell line JURKAT
(Immunofluorescent staining of human cell line JURKAT shows localization to nuclear bodies & nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70692/1970_H11_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line JURKAT shows localization to nuclear bodies & nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70692/1970_H11_1_blue_red_green.jpg
Figure 4 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies & nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70692/1933_A12_8_cr5d1223636ae4e_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies & nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70692/1933_A12_8_cr5d1223636ae4e_blue_red_green.jpg
Figure 5 Cerebral cortex
(Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9656/24924_B_9_5.jpg
(Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9656/24924_B_9_5.jpg
Figure 6 Liver
(Hepatocytes
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9656/24924_A_8_4.jpg
(Hepatocytes
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9656/24924_A_8_4.jpg
Figure 7 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9656/24924_A_9_5.jpg
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9656/24924_A_9_5.jpg
Figure 8 Testis
(Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9656/24924_A_6_6.jpg
(Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9656/24924_A_6_6.jpg
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000103343-ZNF174
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000103343-ZNF174
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000103343-ZNF174
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000103343-ZNF174
Specifications
- Immunogen
- Recombinant full length protein, corresponding to amino acids 1-235 of Human ZNF174
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- EML2238
- Applications
- Used for immunoassay techniques such as: Immunocytochemistry/Immunofluorescence; Western Blot
- Conjugate
- Unconjugated
Product Property
- Purity
- Protein G purified
- Format
- Liquid
- Concentration
- 1.0 mg/ml
- Buffer
- Preservative: None PBS, pH 7.2
- Storage
- Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- ZNF174; zinc finger protein 174; ZSCAN8; AW-1; zinc finger and SCAN domain-containing protein 8;
- Gene ID
- 7727
- UniProt ID
- Q15697
- Protein Refseq
- NP_001027463
- Function
- DNA binding; metal ion binding; protein homodimerization activity; sequence-specific DNA binding transcription factor activity; transcription regulatory region DNA binding; zinc ion binding;
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Fab Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-736 | Human Anti-ZNF174 Recombinant Antibody (clone D578); Fab Fragment | IP, IF, ELISA | Human Fab |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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