Rabbit Anti-IGHM Recombinant Antibody (clone DS1775AB)
CAT#: MOR-1775
Recombinant Rabbit anti-IGHM monoclonal antibody for WB, IP, IHC-P, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Normal Tissue
Figure 1 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8643/24789_A_9_3.jpg
Normal Tissue
Figure 2 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8643/24789_A_9_8.jpg
RNA Expression
Figure 3 RNA cell line category: Group enriched (Daudi, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000211899-IGHM
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Specifications
- Immunogen
- IgM from Human serum.
- Host Species
- Rabbit
- Derivation
- Phage display library
- Type
- Rabbit IgG
- Specificity
- Human IGHM
- Species Reactivity
- Human
- Clone
- DS1775AB
- Applications
- WB, IP, IHC-P
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Superior Recombinant Rabbit Antibody
This recombinant rabbit monoclonal antibody (clone DS1775AB) is superior to traditional reagents. We used it for IP and Western Blot, and it showed excellent affinity for human IGHM. The background was minimal, which is great.
Perfect For IHC-P
We applied this antibody to paraffin-embedded sections to detect IgM. The results were sharp and specific. Being derived from a phage display library, it seems to have been selected for high specificity and performance.
Q&As
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How is this recombinant antibody developed?
A: This Hi-Affi™ antibody is developed using phage display library technology. This approach allows us to avoid traditional ascitic fluid methods, producing a high-specificity recombinant rabbit antibody against Human IGHM that is humane and consistent.
-
Can this antibody be used for paraffin-embedded tissues?
A: Yes, this antibody (clone DS1775AB) is excellent for Immunohistochemistry on paraffin-embedded sections (IHC-P). It is also recommended for Western Blot (WB) and Immunoprecipitation (IP) to detect IgM heavy chains.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "IGHM"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1079z | Mouse Anti-IGHM Recombinant Antibody (clone 18C5) | FC, FuncS, ICC, IF, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0795-CN | Mouse Anti-IGHM Recombinant Antibody (clone B6-8) | WB, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0796-CN | Mouse Anti-IGHM Recombinant Antibody (clone 2-B5) | WB, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0797-CN | Mouse Anti-IGHM Recombinant Antibody (clone 4-B2) | WB, IHC, ELISA | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0798-CN | Mouse Anti-IGHM Recombinant Antibody (clone 9-C1) | WB, IHC, ELISA | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3462 | Anti-IGHM Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3463 | Anti-Mouse IGHM Immunohistochemistry Kit | IHC |
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