Anti-Human ING4 Therapeutic Antibody (BTIM-4)

Figure 1 Generation and characterization of a monoclonal anti-ING4 antibody.

Detection of ING4 and HA-epitope tagged ING4 overexpressed in 293T cells by Western blot using BTIM-4 and anti-HA monoclonal antibody. * denotes smaller ING4-derived protein species recognized by the antibody.

Byron, S. A., Min, E., Thal, T. S., Hostetter, G., Watanabe, A. T., Azorsa, D. O., ... & Kim, S. (2012). Negative regulation of NF-κB by the ING4 tumor suppressor in breast cancer. PloS one, 7(10), e46823.

Figure 2 Generation and characterization of a monoclonal anti-ING4 antibody.

MCF10A cells transduced with pMIG (the vector control), ING4 (ING4 overexpression), shNT (non-targeting shRNA control), or shING4 (shRNA targeting ING4), and T47D cells transduced with pMIG (the vector control) or ING4 (ING4 overexpression) were immunostained with BTIM-4 anti-ING4 antibody (red) and visualized using fluorescent microscopy. 4′,6-Diamidino-2-phenylindole (DAPI) was used to stain individual cell nuclei (blue). White scale bars represent 100 µm.

Byron, S. A., Min, E., Thal, T. S., Hostetter, G., Watanabe, A. T., Azorsa, D. O., ... & Kim, S. (2012). Negative regulation of NF-κB by the ING4 tumor suppressor in breast cancer. PloS one, 7(10), e46823.

Figure 3 Inverse correlation between ING4 and p-p65/RelA (Ser276) antibody staining in breast tumor samples.

(A,B) Hyperplastic breast tissue with moderate atypia from a ductal carcinoma in situ case, showing ING4 score +3 and p-p65 score 0. Inset is a higher magnification image of the region showing ING4 staining in luminal and basal epithelial cells and in stroma cells. (C,D) Invasive ductal carcinoma with ING4 score 0 and p-p65 score +2. (E,F) Grade 3 invasive ductal carcinoma with ING4 score +1 and p-p65 score +3. (bars, 100 µm).

Byron, S. A., Min, E., Thal, T. S., Hostetter, G., Watanabe, A. T., Azorsa, D. O., ... & Kim, S. (2012). Negative regulation of NF-κB by the ING4 tumor suppressor in breast cancer. PloS one, 7(10), e46823.