Anti-Human ING4 Therapeutic Antibody (BTIM-4) (CAT#: TAB-0030CL)

Anti-ING4 monoclonal antibody is an antibody that specifically binds to the Inhibitor of Growth 4 (ING4) protein, which is a member of the ING tumor suppressor family. By binding to the ING4, the anti-ING4 monoclonal antibody can be used to diagnose the cancer, like breast cancer.

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WB

Figure 1 Generation and characterization of a monoclonal anti-ING4 antibody.

Detection of ING4 and HA-epitope tagged ING4 overexpressed in 293T cells by Western blot using BTIM-4 and anti-HA monoclonal antibody. * denotes smaller ING4-derived protein species recognized by the antibody.

Byron, S. A., Min, E., Thal, T. S., Hostetter, G., Watanabe, A. T., Azorsa, D. O., ... & Kim, S. (2012). Negative regulation of NF-κB by the ING4 tumor suppressor in breast cancer. PloS one, 7(10), e46823.

IF

Figure 2 Generation and characterization of a monoclonal anti-ING4 antibody.

MCF10A cells transduced with pMIG (the vector control), ING4 (ING4 overexpression), shNT (non-targeting shRNA control), or shING4 (shRNA targeting ING4), and T47D cells transduced with pMIG (the vector control) or ING4 (ING4 overexpression) were immunostained with BTIM-4 anti-ING4 antibody (red) and visualized using fluorescent microscopy. 4′,6-Diamidino-2-phenylindole (DAPI) was used to stain individual cell nuclei (blue). White scale bars represent 100 µm.

Byron, S. A., Min, E., Thal, T. S., Hostetter, G., Watanabe, A. T., Azorsa, D. O., ... & Kim, S. (2012). Negative regulation of NF-κB by the ING4 tumor suppressor in breast cancer. PloS one, 7(10), e46823.

IHC

Figure 3 Inverse correlation between ING4 and p-p65/RelA (Ser276) antibody staining in breast tumor samples.

(A,B) Hyperplastic breast tissue with moderate atypia from a ductal carcinoma in situ case, showing ING4 score +3 and p-p65 score 0. Inset is a higher magnification image of the region showing ING4 staining in luminal and basal epithelial cells and in stroma cells. (C,D) Invasive ductal carcinoma with ING4 score 0 and p-p65 score +2. (E,F) Grade 3 invasive ductal carcinoma with ING4 score +1 and p-p65 score +3. (bars, 100 µm).

Byron, S. A., Min, E., Thal, T. S., Hostetter, G., Watanabe, A. T., Azorsa, D. O., ... & Kim, S. (2012). Negative regulation of NF-κB by the ING4 tumor suppressor in breast cancer. PloS one, 7(10), e46823.


Specifications

  • Host Species
  • Mouse
  • Specificity
  • Human
  • Clone
  • BTIM-4
  • Applications
  • WB, IF, IHC
  • Related Disease
  • Breast tumor

Applications

  • Application Notes
  • This antibody has been reported for use in Western Blot, Immunofluorescence and Immunohistochemistry.
    Immunohistochemistry: Immunochemical staining of TMA sections was performed using BOND-MAX autostainer. Antibodies used for IHC were anti-estrogen receptor-alpha (ER-α) monoclonal (1∶200), anti-HER2/neu polyclonal (1∶300), anti-phospho-p65 (Ser276) polyclonal (1∶40), and anti-ING4 monoclonal (1∶2, BTIM-4 cell culture supernatant). The staining intensity of estrogen receptor (ER) and HER2 was scored as described previously. All TMA and whole sections were scored manually by a board certified pathologist. An IHC score was assigned to each sample by averaging the scores of double or triple punch samples. The percent of evaluable IHC staining on TMAs ranged from 94.5% (ING4) to 99% (p-p65).

Target

  • Alternative Names
  • ING4; inhibitor of growth family, member 4; my036; p29ING4; inhibitor of growth protein 4; brain my036 protein; candidate tumor suppressor p33 ING1 homolog

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

See other products for "ING4"

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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