Mouse Anti-Influenza A Virus Hemagglutinin H3 HA Recombinant Antibody (MRO-280LC) (CAT#: MRO-280LC)
This product is a recombinant mouse antibody that recognizes HA. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
Figure 1 Anti-Influenza A Virus Hemagglutinin H3 HA Recombinant Antibody (MRO-280LC) in SDS-PAGE
SDS-PAGE analysis of MRO-280LC in non-reduced (Lane 1) and β-mercaptoethanol-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 180-245 kDa.
Figure 2 Anti-Influenza A Virus Hemagglutinin H3 HA Recombinant Antibody (MRO-280LC) in SEC-HPLC
SEC-HPLC analysis of MRO-280LC shows that the purity is more than 95%.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
Figure 3 Anti-Influenza A Virus Hemagglutinin H3 HA Recombinant Antibody (MRO-280LC) in ELISA
ELISA analysis of MRO-280LC was performed by coating with Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA Protein (His Tag). Then blocked with BSA and incubated with MRO-280LC. The HRP-conjugated goat anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 4 Anti-Influenza A Virus Hemagglutinin H3 HA Recombinant Antibody (MRO-280LC) in ELISA
ELISA analysis of MRO-280LC was performed by coating with Influenza A H3N2 (A/Victoria/210/2009) Hemagglutinin / HA Protein (His Tag). Then blocked with BSA and incubated with MRO-280LC. The HRP-conjugated goat anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 5 Anti-Influenza A Virus Hemagglutinin H3 HA Recombinant Antibody (MRO-280LC) in WB
Western blot analysis of MRO-280LC was performed with Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with MRO-280LC and HRP goat anti-Mouse IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.8 μg)
Figure 6 Anti-Influenza A Virus Hemagglutinin H3 HA Recombinant Antibody (MRO-280LC) in WB
Western blot analysis of MRO-280LC was performed with Influenza A H3N2 (A/Victoria/210/2009) Hemagglutinin / HA Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with MRO-280LC and HRP goat anti-Mouse IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.8 μg)
Figure 7 Anti-Influenza A Virus Hemagglutinin H3 HA Recombinant Antibody (MRO-280LC) in DB
Dot Blot analysis of MRO-280LC was performed by coating with Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA Protein (His Tag).
MRO-280LC incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Mouse IgG as a secondary antibody (1: 2000)
Figure 8 Anti-Influenza A Virus Hemagglutinin H3 HA Recombinant Antibody (MRO-280LC) in DB
Dot Blot analysis of MRO-280LC was performed by coating with Influenza A H3N2 (A/Victoria/210/2009) Hemagglutinin / HA Protein (His Tag).
MRO-280LC incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Mouse IgG as a secondary antibody (1: 2000)
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Influenza A virus hemagglutinin H3
- Applications
- WB, ELISA, IF
- Related Disease
- Influenza virus infections
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- See the COA
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Influenza A Virus Hemagglutinin H3 HA
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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(Creative Biolabs Cat# MRO-280LC, RRID: AB_3111538)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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