Hi-Affi™ Recombinant Rabbit Anti-KIF1A Monoclonal Antibody (DS1935AB)
CAT#: MOR-1935
Recombinant Rabbit anti-KIF1A monoclonal antibody for WB, ICC/IF, FC, IP, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to cytosol & centriolar satellites.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5442/1698_H1_1_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Cell line enhanced (AN3-CA, HAP1, SCLC-21H, SH-SY5Y)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000130294-KIF1A
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Specifications
- Immunogen
- Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human KIF1A aa 900-1000. The exact sequence is proprietary.
- Host Species
- Rabbit
- Derivation
- Phage display library screening
- Type
- IgG
- Species Reactivity
- Rat, Human
- Clone
- DS1935AB
- Applications
- Used for immunoassay techniques such as: Western Blot; Immunocytochemistry/Immunofluorescence; Flow Cytometry; Immunoprecipitation
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- > 95% as determined by both RP-HPLC and SDS-PAGE analysis.
- Format
- Liquid
- Storage
- Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.
Target
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Superior WB Sensitivity
The Hi-Affi™ technology really makes a difference. We used this recombinant rabbit monoclonal antibody for Western Blotting, and the sensitivity was superior to other antibodies we've tried. It detected KIF1A cleanly and with high intensity. It is also excellent for immunocytochemistry applications.
Consistent Recombinant Performance
We appreciate that this is a recombinant antibody derived from phage display. It avoids the variability of traditional methods. We used it for Flow Cytometry and Immunoprecipitation, and the results were consistent and reliable. It specifically targets the KIF1A motor protein, which is vital for our axonal transport studies.
Q&As
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What is Hi-Affi™ technology?
A: Hi-Affi™ antibodies, like this anti-KIF1A, are recombinant monoclonal antibodies developed to provide superior results. They are derived from sources like phage display libraries and produced recombinantly to ensure high specificity, sensitivity, and lot-to-lot consistency, avoiding the variability of traditional hybridomas.
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Can this antibody be used for Immunoprecipitation?
A: Yes, this Recombinant Rabbit Monoclonal Antibody is validated for Immunoprecipitation (IP), as well as Western Blot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), and Flow Cytometry (FC). It is purified with Protein A Agarose to ensure optimal performance in these assays.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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