Hi-Affi™ Recombinant Rabbit Anti-MARCKSL1 Monoclonal Antibody (DS2166AB)
CAT#: MOR-2166
Recombinant Rabbit anti-MARCKSL1 monoclonal antibody for WB, IHC-P, ICC/IF, FC, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30528/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20787/if_selected.jpg
Normal Tissue
Figure 3 Breast
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Myoepithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30528/134910_B_2_4.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30528/134910_A_9_8.jpg
Normal Tissue
Figure 5 Cerebral cortex
Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30528/134910_B_7_5.jpg
Normal Tissue
Figure 6 Colon
Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30528/134910_A_8_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30528/134910_A_9_4.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (AF22, NTERA-2)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000175130-MARCKSL1
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Specifications
- Immunogen
- Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MARCKS like protein aa 150 to the C-terminus. The exact sequence is proprietary.
- Host Species
- Rabbit
- Derivation
- Phage display library screening
- Type
- IgG
- Species Reactivity
- Mouse, Rat, Human
- Clone
- DS2166AB
- Applications
- Used for immunoassay techniques such as: Western Blot; Immunohistochemistry (Paraffin); Immunocytochemistry/Immunofluorescence; Flow Cytometry
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- > 95% as determined by both RP-HPLC and SDS-PAGE analysis.
- Format
- Liquid
- Storage
- Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "MARCKSL1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1206CT | Recombinant Mouse anti-Human MARCKSL1 Monoclonal antibody (EML1767) | WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1305 | Anti-MARCKSL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4260 | Anti-Human MARCKSL1 Immunohistochemistry Kit | IHC |
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