Mouse Anti-MCM6 Recombinant Antibody (TAB-0742CL)
CAT#: TAB-0742CL
This product is a recombinant Mouse antibody that recognizes mini-chromosome maintenance protein 6. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
Gene Expression
Normal Tissue
Figure 1 Tonsil
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4818/14021_A_6_8_val_selected.jpg
Normal Tissue
Figure 2 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4818/14021_A_9_8.jpg
Normal Tissue
Figure 3 Colon
Endocrine cells Staining: Medium Intensity: Moderate Enterocytes Staining: Medium Intensity: Strong Quantity: <25% Enterocytes - Gradient Gradient: Descending Enterocytes - Goblet cells Staining: Medium Intensity: Strong Quantity: <25% Mucosal lymphoid cells Staining: Low Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4818/14021_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Collecting ducts Staining: Low Intensity: Moderate Quantity: <25% Distal tubules Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4818/14021_A_9_5.jpg
Normal Tissue
Figure 5 Testis
Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4818/14021_A_6_6.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000076003-MCM6
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Specifications
- Immunogen
- Recombinant FLAG-tagged MCM6
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human MCM6
- Species Reactivity
- Human
- Applications
- ELISA, ICC, WB, IHC
- Related Disease
- Cervical disease
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Detects MCM6 In Cells
We used this recombinant mouse antibody for immunocytochemistry to visualize MCM6 in the nucleus. It gave a clear signal and worked well in Western Blot as well. The product was pure and stable.
Reliable For ELISA
This antibody performed reliably in our ELISA screening for MCM6 levels. It recognizes the human mini-chromosome maintenance protein 6 specifically. We appreciate the chemically defined culture media used for its expression.
Q&As
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What expression system is used for this antibody?
A: This recombinant mouse antibody is expressed in mammalian cells using chemically defined culture media. This system is chosen to ensure proper folding and post-translational modifications, resulting in a high-quality antibody for recognizing human MCM6.
-
Is this antibody suitable for detecting MCM6 in the nucleus?
A: Yes, MCM6 is a nuclear protein involved in genome replication. This antibody has been validated for Immunocytochemistry (ICC) and Immunohistochemistry (IHC), where it can be used to visualize the localization of MCM6 in the cell nucleus.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "MCM6"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0742CL-S(P) | Mouse Anti-MCM6 Recombinant Antibody; scFv Fragment (TAB-0742CL-S(P)) | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0742CL-F(E) | Mouse Anti-MCM6 Recombinant Antibody; Fab Fragment (TAB-0742CL-F(E)) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-FY912 | Recombinant Rabbit Anti-MCM6 Antibody (clone R02-2I2) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1323 | Anti-MCM6 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC715 | Rabbit Anti-MCM6 Recombinant Antibody (VS13-YC715) | IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4312 | Anti-Mouse MCM6 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4311 | Anti-Human MCM6 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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