Human Anti-MG Recombinant Antibody (clone L5*); scFv Fragment (CAT#: PSBZ-156)
Recombinant Human Antibody (L5*) is capable of binding to MG (malachite green), expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-MG (malachite green) scFv and CH1-3 region of human IgG and a light chain (LC) encoding VL from anti-MG (malachite green) proteins scFv and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. The L5* FAP studied here is a leucine-to-serine point mutant (Ser89) obtained by directed evolution of L5, which is a VL single domain antibody fragment selected from the original yeast display library.
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Normal Tissue
Figure 1 IHC staining of human duodenum
(High expression. RNA expression: 58.9 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2270/8420_A_9_2_val_selected.jpg
(High expression. RNA expression: 58.9 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2270/8420_A_9_2_val_selected.jpg
Figure 2 IHC staining of human liver
(Low expression. RNA expression: 0.5 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2270/8420_A_7_4_val_selected.jpg
(Low expression. RNA expression: 0.5 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2270/8420_A_7_4_val_selected.jpg
Figure 3 Cerebral cortex
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2270/8420_B_8_5.jpg
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2270/8420_B_8_5.jpg
Figure 4 Small intestine
(Enterocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Enterocytes - Microvilli
Staining: High
Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2270/8420_A_6_2.jpg
(Enterocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Enterocytes - Microvilli
Staining: High
Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2270/8420_A_6_2.jpg
Figure 5 Colon
(Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2270/8420_A_9_3.jpg
(Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2270/8420_A_9_3.jpg
Figure 6 Kidney
(Proximal tubules (microvilli)
Staining: High
Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2270/8420_A_7_5.jpg
(Proximal tubules (microvilli)
Staining: High
Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2270/8420_A_7_5.jpg
Figure 7 Testis
(Elongated or late spermatids
Staining: Low
Intensity: Moderate
Quantity: <25%
Round or early spermatids
Staining: Low
Intensity: Moderate
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2270/8420_A_5_6.jpg
(Elongated or late spermatids
Staining: Low
Intensity: Moderate
Quantity: <25%
Round or early spermatids
Staining: Low
Intensity: Moderate
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2270/8420_A_5_6.jpg
Figure 8 RNA cell line category: Cell line enhanced (A549, BJ hTERT+, HL-60, U-937, WM-115)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000257335-MGAM
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000257335-MGAM
Specifications
- Immunogen
- Malachite green
- Host Species
- Human
- Derivation
- Human
- Type
- Human scFv
- Clone
- L5*
- Applications
- WB
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- MG; malachite green
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibodies
Fab Antibodies
ScFv Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-658 | Recombinant Anti-MG Antibody | WB, IHC, FuncS | IgG |
| MHH-658 | Recombinant Human Anti-MG Antibody | WB, IP, FuncS | IgG |
| PABZ-156 | Human Anti-MG Recombinant Antibody (clone L5*) | WB | Human IgG |
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