Human Anti-MG Recombinant Antibody (clone L5*); Fab Fragment (CAT#: PFBZ-156)
Recombinant Human Antibody (L5*) is capable of binding to MG (malachite green), expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-MG (malachite green) fab and CH1-3 region of human IgG and a light chain (LC) encoding VL from anti-MG (malachite green) proteins fab and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. The L5* FAP studied here is a leucine-to-serine point mutant (Ser89) obtained by directed evolution of L5, which is a VL single domain antibody fragment selected from the original yeast display library.
Specific Inquiry
Normal Tissue
Figure 1 IHC staining of human duodenum
(High expression. RNA expression: 58.9 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2270/8420_A_9_2_val_selected.jpg
(High expression. RNA expression: 58.9 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2270/8420_A_9_2_val_selected.jpg
Figure 2 IHC staining of human liver
(Low expression. RNA expression: 0.5 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2270/8420_A_7_4_val_selected.jpg
(Low expression. RNA expression: 0.5 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2270/8420_A_7_4_val_selected.jpg
Figure 3 Cerebral cortex
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2270/8420_B_8_5.jpg
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2270/8420_B_8_5.jpg
Figure 4 Small intestine
(Enterocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Enterocytes - Microvilli
Staining: High
Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2270/8420_A_6_2.jpg
(Enterocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Enterocytes - Microvilli
Staining: High
Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2270/8420_A_6_2.jpg
Figure 5 Colon
(Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2270/8420_A_9_3.jpg
(Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2270/8420_A_9_3.jpg
Figure 6 Kidney
(Proximal tubules (microvilli)
Staining: High
Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2270/8420_A_7_5.jpg
(Proximal tubules (microvilli)
Staining: High
Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2270/8420_A_7_5.jpg
Figure 7 Testis
(Elongated or late spermatids
Staining: Low
Intensity: Moderate
Quantity: <25%
Round or early spermatids
Staining: Low
Intensity: Moderate
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2270/8420_A_5_6.jpg
(Elongated or late spermatids
Staining: Low
Intensity: Moderate
Quantity: <25%
Round or early spermatids
Staining: Low
Intensity: Moderate
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2270/8420_A_5_6.jpg
Figure 8 RNA cell line category: Cell line enhanced (A549, BJ hTERT+, HL-60, U-937, WM-115)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000257335-MGAM
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000257335-MGAM
Specifications
- Immunogen
- Malachite green
- Host Species
- Human
- Derivation
- Human
- Type
- Human Fab
- Clone
- L5*
- Applications
- WB
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- MG; malachite green
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Fab Antibody Products
ScFv Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-658 | Recombinant Anti-MG Antibody | WB, IHC, FuncS | IgG |
| MHH-658 | Recombinant Human Anti-MG Antibody | WB, IP, FuncS | IgG |
| PABZ-156 | Human Anti-MG Recombinant Antibody (clone L5*) | WB | Human IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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