Recombinant Mouse Anti-MK2-23 idiotype Antibody scFv Fragment (GH1002)

There are eight asymmetric units in this unit cell, each formed by one Fab. The two 2-fold crystallographically related Fab fragments forming a complex are colored in red, green, blue and yellow, and the coordinate axes are in red. The Figure was generated with ''O'' (Jones & Kjeldgaard, 1990), and displayed on an SGI 320 VGX workstation.

Ban, N., Day, J., Wang, X., Ferrone, S., & McPherson, A. (1996). Crystal structure of an anti-anti-idiotype shows it to be self-complementary. Journal of molecular biology, 255(4), 617-627.

Two different measurements are combined in this Figure. The distribution indicated by a broken line was obtained by measuring a solution containing a Fab fragment of an anti-anti-idiotopic mAb GH1002; as a control, the continuous line represents the distribution of mass in a solution containing a Fab fragment of idiotopic mAb 763-74. The average hydrodynamic radius of particles is 69A° and 51A°, respectively, corresponding to a dimer formed by a head-to-head interaction of two Fab fragments and a monomer. The
measurement was performed for a duration of 60 seconds at 295 K.

Ban, N., Day, J., Wang, X., Ferrone, S., & McPherson, A. (1996). Crystal structure of an anti-anti-idiotype shows it to be self-complementary. Journal of molecular biology, 255(4), 617-627.

The model bias was minimized by refining the model lacking CDRs and subsequently calculating the map. CDR loops are stick model in white, the rest of the molecule is shown as a Ca trace, and symmetry-related atoms are in red. The Figure was generated with ''O'' (Jones & Kjeldgaard, 1990), and displayed on an SGI 320 VGX workstation.

Ban, N., Day, J., Wang, X., Ferrone, S., & McPherson, A. (1996). Crystal structure of an anti-anti-idiotype shows it to be self-complementary. Journal of molecular biology, 255(4), 617-627.