This clone mAb TCC56 was secreted from hybridoma and then was produced as a recombinant antibody, which was an IgG3κ isotype. Biotinylated proteins were at 35 KDa by immunoprecipitation using mAb TCC56 under reducing conditions. MUC13 was identified as a possible candidate for the 35 KDa molecule by mass spectrometry. Flow cytometry results indicated that mAb TCC56 reacted with transfectants expressing MUC13.
Figure 1 Lysates of TCC-PAN2 cells were immunoprecipitated with mAb TCC56.
Nishii, Y., Yamaguchi, M., Kimura, Y., Hasegawa, T., Aburatani, H., Uchida, H., ... & Sakuma, Y. (2015). A newly developed anti-Mucin 13 monoclonal antibody targets pancreatic ductal adenocarcinoma cells. International journal of oncology, 46(4), 1781-1787.
Figure 2 Flow cytometry results indicate the reactivity of mAb TCC56 or P3 (a negative control mouse IgG1) with CHO-K1 cells transfected with a MUC13 expression vector. The mAb TCC56 reacted with transfectants expressing MUC13.
Nishii, Y., Yamaguchi, M., Kimura, Y., Hasegawa, T., Aburatani, H., Uchida, H., ... & Sakuma, Y. (2015). A newly developed anti-Mucin 13 monoclonal antibody targets pancreatic ductal adenocarcinoma cells. International journal of oncology, 46(4), 1781-1787.
Figure 3 Flow cytometry results shows the reactivity of mAb TCC56 with TCC-PAN 2 cells.
Nishii, Y., Yamaguchi, M., Kimura, Y., Hasegawa, T., Aburatani, H., Uchida, H., ... & Sakuma, Y. (2015). A newly developed anti-Mucin 13 monoclonal antibody targets pancreatic ductal adenocarcinoma cells. International journal of oncology, 46(4), 1781-1787.
Figure 4 mAb TCC56-DT3C conjugate induced TCC-PAN2 cell death.
Nishii, Y., Yamaguchi, M., Kimura, Y., Hasegawa, T., Aburatani, H., Uchida, H., ... & Sakuma, Y. (2015). A newly developed anti-Mucin 13 monoclonal antibody targets pancreatic ductal adenocarcinoma cells. International journal of oncology, 46(4), 1781-1787.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-440MZ-S(P) | Anti-Human MUC13 Recombinant Antibody scFv Fragment (TCC56) | IP | |
TAB-440MZ-F(E) | Anti-Human MUC13 Recombinant Antibody Fab Fragment (TCC56) | IP |
There are currently no Customer reviews or questions for TAB-440MZ. Click the button above to contact us or submit your feedback about this product.
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-440MZ, RRID: AB_3111946)
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.