Recombinant Mouse Antibody (MN16C13F4) is capable of binding to N. meningitidis PorA (P1. 2), expressed in Chinese Hamster Ovary cells (CHO).
Figure 1 Binding of anti-rNspA and control MAbs to live encapsulated N. meningitidis serogroup B strains BZ198, 8047, and M986 as determined by indirect-fluorescence flow cytometry.
The anti-NspA MAbs include AL4, AL5, AL11, and AL12. The control MAbs include a murine MAb with an irrelevant specificity (VIG10), an anticapsular-specific murine MAb (SEAM 12), and, for strains 8047 and M986, an N. meningitidis subtype MAb anti-PorA P1.2 (Rijksinstituut Voor Volksgezondheid en Mileu, Bilthoven, The Netherlands). A subtyping anti-PorA MAb was not available for strain BZ198.
Moe, G. R., Zuno-Mitchell, P., Lee, S. S., Lucas, A. H., & Granoff, D. M. (2001). Functional activity of anti-neisserial surface protein A monoclonal antibodies against strains of Neisseria meningitidis serogroup B. Infection and immunity, 69(6), 3762-3771.
Figure 2 The SDS-PAGE-separated proteins of strain 15501 (known to express both PorA and PorB) were transferred to nitrocellulose (lanes 1 and 2).
Both lanes were cut at the arrows. The left half of lane 1 was incubated with a mAb specific for PorA P1.2. The right half of lane 1 was incubated with MBL, as was the left half of lane 2. The right half of lane 2 was incubated with a mAb specific for PorB 2a. MBL bound to the same band as the PorB mAb, indicating that PorB but not PorA bound MBL.
Estabrook, M. M., Jack, D. L., Klein, N. J., & Jarvis, G. A. (2004). Mannose-binding lectin binds to two major outer membrane proteins, opacity protein and porin, of Neisseria meningitidis. The Journal of Immunology, 172(6), 3784-3792.
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CAT | Product Name | Application | Type |
---|---|---|---|
PABZ-091 | Recombinant Mouse Anti-N. meningitidis P1.7 peptide Antibody (MN14C11.6) | ELISA | IgG |
PABW-091 | Recombinant Mouse Anti-N. meningitidis PorA Antibody (MN12H2) | WB, ELISA | IgG |
PABC-092 | Recombinant Mouse Anti-N. meningitidis porA Antibody (MN20B9.34) | WB, ELISA | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBZ-091 | Recombinant Mouse Anti-N. meningitidis P1.7 peptide Antibody Fab Fragment (MN14C11.6) | ELISA | IgG |
PFBW-091 | Recombinant Mouse Anti-N. meningitidis PorA Antibody Fab Fragment (MN12H2) | WB, ELISA | IgG |
PFBC-092 | Recombinant Mouse Anti-N. meningitidis porA Antibody Fab Fragment (MN20B9.34) | WB, ELISA | IgG |
PFBW-092 | Recombinant Mouse Anti-N. meningitidis PorA (P1.2) Antibody Fab Fragment (MN16C13F4) | ELISA | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBZ-091 | Recombinant Mouse Anti-N. meningitidis P1.7 peptide Antibody scFv Fragment (MN14C11.6) | ELISA | scFv |
PSBW-091 | Recombinant Mouse Anti-N. meningitidis PorA Antibody scFv Fragment (MN12H2) | WB, ELISA | scFv |
PSBC-092 | Recombinant Mouse Anti-N. meningitidis porA Antibody scFv Fragment (MN20B9.34) | WB, ELISA | scFv |
PSBW-092 | Recombinant Mouse Anti-N. meningitidis PorA (P1.2) Antibody scFv Fragment (MN16C13F4) | ELISA | scFv |
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