Anti-P. aeruginosa PsL Antibody.

Customer Review

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Emily Roberts
12.Sep,23

Excellent for PsL Detection

The Anti-P. aeruginosa PsL Antibody has been excellent for our Pseudomonas detection studies. Its specificity and sensitivity are top-notch, providing clear and reliable results. This antibody has significantly improved the accuracy of our experiments and saved us a lot of troubleshooting time.

Brian Anderson
21.Oct,22

Effective in Immunoassays

We incorporated this antibody into our immunoassays, and it has been a great asset. The specificity for PsL is precise, allowing for accurate detection and analysis. The reliable performance has made it an invaluable tool in our lab.

Jessica Moore
17.May,21

Consistent and High-Quality

This antibody has proven to be consistent and high-quality in our assays. The reproducible results and strong affinity for PsL have significantly improved our experiments, making it a crucial part of our research toolkit. The consistent performance has greatly enhanced our data quality.

Q&As

1. Is the anti-Pseudomonas aeruginosa PsL antibody suitable for use in Western blotting?

   A: Yes, the anti-Pseudomonas aeruginosa PsL antibody (MOB-0286MC) is suitable for use in Western blotting. It provides specific binding to PsL, allowing for reliable detection in Western blot assays.

2. What are the storage recommendations for the anti-Pseudomonas aeruginosa PsL antibody?

   A: The recommended storage condition for the anti-Pseudomonas aeruginosa PsL antibody (MOB-0286MC) is at -20°C or lower. For short-term storage, it can be kept at 2-8°C. To ensure stability, avoid repeated freeze-thaw cycles.

3. Can the anti-Pseudomonas aeruginosa PsL antibody be used in immunoprecipitation assays?

   A: Yes, the anti-Pseudomonas aeruginosa PsL antibody (MOB-0286MC) can be used in immunoprecipitation assays. It provides specific binding to PsL, enabling the successful precipitation of the target protein from complex mixtures.

4. Is the anti-Pseudomonas aeruginosa PsL antibody effective in ELISA applications?

   A: Yes, the anti-Pseudomonas aeruginosa PsL antibody (MOB-0286MC) is effective in ELISA applications. It has been validated for use in such assays and provides reliable detection of PsL.

5. What is the optimal dilution for using the anti-Pseudomonas aeruginosa PsL antibody in immunofluorescence?

   A: The optimal dilution for using the anti-Pseudomonas aeruginosa PsL antibody (MOB-0286MC) in immunofluorescence is typically 1:100 to 1:500. It is advisable to perform a dilution series to determine the best working concentration for your specific experimental conditions.

View the frequently asked questions answered by Creative Biolabs Support.

Citations

1. Stoner, Sara N., Joshua J. Baty, and Jessica A. Scoffield. "Pseudomonas aeruginosa polysaccharide Psl supports airway microbial community development." The ISME journal 16.7 (2022): 1730-1739. https://doi.org/10.1038/s41396-022-01221-y
   This study explores the role of Pseudomonas aeruginosa polysaccharide Psl in the development of airway microbial communities, specifically within the context of cystic fibrosis (CF). The research focuses on interactions between P. aeruginosa and Streptococcus salivarius, demonstrating that Psl promotes biofilm formation by S. salivarius. The study employs both in vitro and in vivo models to show that S. salivarius uses a maltose-binding protein, MalE, to interact with Psl, facilitating its incorporation into the CF airway biofilm and potentially contributing to lung stability in CF patients.
   Creative Biolabs provided the FITC-conjugated α-Psl antibody (Cat#: MOB-0286MC) used in the immunofluorescence and confocal laser scanning microscopy experiments. This antibody was crucial for visualizing the spatial relationship between Psl and S. salivarius within dual biofilms, allowing researchers to confirm the role of Psl in promoting S. salivarius biofilm formation and understanding the structural changes in the biofilm architecture.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# MOB-0286MC, RRID: AB_3111473)

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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