Afuco™ Anti-Human PS ADCC Recombinant Antibody (PGN635), ADCC Enhanced
CAT#: AFC-745CL
Anti-PS ADCC Enhanced Antibody (PGN635) is an ADCC enhanced antibody produced by our Afuco™ platform. PGN635 is a novel monoclonal antibody that binds PS. PGN635 may be a useful new imaging probe for detection and therapy for tumor.
Published Data
ELISA
Figure 7 Radioiodination does not affect binding of PGN635 F(ab’)2 to PS.
A) Competition ELISA shows 125I- PGN635 F(ab')2 and unlabeled PGN635 F(ab')2 have similar ability to compete with PGN635-biotin for binding to immobilized PS. B) Binding of ̶¹²⁴I-PGN635 F(ab')2 to PS-expressing cells. PS-exposure on ABAE and PC3-luc cells was induced by irradiation with 5 Gy 24 h before the binding assay. Non-irradiated cells served as controls. The cells were incubated with ̶¹²⁴I-PGN635 F(ab')2 or ̶¹²⁴I-control F(ab')2 for 1 h. The cells were then washed and dissolved in NaOH (1N) and 124I was quantified by gamma counting (CPM = counts/min.). Points and histograms, means; bars, + S.D.
Stafford, J. H., Hao, G., Best, A. M., Sun, X., & Thorpe, P. E. (2013). Highly specific PET imaging of prostate tumors in mice with an iodine-124-labeled antibody fragment that targets phosphatidylserine. PLoS one, 8(12), e84864.
Figure 8 ¹²⁵I-PGN635 F(ab’)2 biodistribution.
Mice (n =3) bearing s.c. PC3-luc tumors were injected with 1.85 MBq (50 µg) of ¹²⁵I-PGN635 F(ab')2 or ¹²⁵I-control F(ab')2. Antibody distribution to the indicated organs was determined after 48 h by counting the radioactivity with a gamma counter. A) Biodistribution by percent injected dose per gram (%ID/g) of tissue. B) Biodistribution by percent injected dose per organ (%ID/organ). The % ID in the blood was calculated assuming a blood volume of 2.18ml/25 g body weight.
Stafford, J. H., Hao, G., Best, A. M., Sun, X., & Thorpe, P. E. (2013). Highly specific PET imaging of prostate tumors in mice with an iodine-124-labeled antibody fragment that targets phosphatidylserine. PLoS one, 8(12), e84864.
FuncS
Figure 9 ¹²⁴I-PGN635 F(ab’)2 localization predicts tumor response to therapy.
A) Mice bearing PC3-luc tumors were treated with 10 mg/kg docetaxel (CTx) or their tumors were irradiated with 15 Gy (RTx). After 24 h, the mice were injected with ¹²⁴I-PGN635 F(ab')2 and imaged by PET 48 h later. B) CTx and RTx significantly inhibited tumor growth (one-way ANOVA, P < 0.01). C) ¹²⁴I-PGN635 F(ab')2 uptake was significantly higher in treated tumors (one-way ANOVA, P < 0.05). D) The tumor-to-normal (T/N) ratio inversely correlated with the tumor growth over the next 28 days (Pearson's r = -0.85, P < 0.01). The tumor volume on day 28 was divided by the volume on the day of treatment to calculate the fold change in tumor volume. The T/N ratio at the time of imaging was predictive of the tumor response.
Stafford, J. H., Hao, G., Best, A. M., Sun, X., & Thorpe, P. E. (2013). Highly specific PET imaging of prostate tumors in mice with an iodine-124-labeled antibody fragment that targets phosphatidylserine. PLoS one, 8(12), e84864.
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Specifications
- Host Species
- Human
- Derivation
- Human
- Type
- ADCC enhanced antibody
- Species Reactivity
- Human
- Applications
- ELISA, IF, IHC, FuncS
- Related Disease
- Cancer
Product Property
- Purity
- 90–95% (SDS-PAGE)
- Storage
- Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
Applications
- Application Notes
- The antibody was validated for ELISA, Immunofluorescence, Immunohistochemistry, Function Assay. For details, refer to Published Data.
Target
- Alternative Names
- PS; Phosphatidylserine; Ptd-L-Ser
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Enhanced ADCC Activity
This antibody from the Afuco platform is impressive. We are studying ADCC mechanisms, and this enhanced version (PGN635) showed superior activity compared to standard antibodies. It binds specifically to Phosphatidylserine (PS). It is a powerful tool for tumor imaging and therapy research.
Validated For Multiple Uses
We utilized this antibody for Immunofluorescence and ELISA. It consistently detected PS exposure on cells. The data provided by the manufacturer was accurate, and our results matched the published data. It is a high-quality, specialized reagent for cancer research.
Q&As
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What does "ADCC Enhanced" mean for this antibody?
A: This Anti-PS Antibody (PGN635) is produced using our Afuco™ platform to be defucosylated. This modification significantly enhances its Antibody-Dependent Cellular Cytotoxicity (ADCC) activity compared to conventional antibodies.
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What does this antibody target?
A: It targets Phosphatidylserine (PS), a phospholipid often exposed on the surface of apoptotic cells and tumor vasculature. It is used for ELISA, IF, IHC, and Functional Studies.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| MOB-819 | Recombinant Anti-PS Antibody | ELISA, WB, Dot, FuncS | IgG |
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| MOB-819-F(E) | Recombinant Anti-PS Antibody Fab Fragment | ELISA, IP, FuncS | Fab |
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| MOB-819-S(P) | Recombinant Anti-PS Antibody scFv Fragment | ELISA, IF, FuncS | scFv |
| CAT | Product Name | Application | Type |
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| MHH-819 | Recombinant Human Anti-PS Antibody | WB, IP, FuncS | IgG |
| CAT | Product Name | Application | Type |
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| MHH-819-F(E) | Recombinant Human Anti-PS Antibody Fab Fragment | WB, Dot, FuncS | Fab |
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| MHH-819-S(P) | Recombinant Human Anti-PS Antibody scFv Fragment | WB, Neut, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABZ-170 | Human Anti-PS Recombinant Antibody (clone pSTAb) | ELISA, WB | Humanized IgG |
| CAT | Product Name | Application | Type |
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| PABZ-172 | Human Anti-PS Recombinant Antibody (clone pSAb) | ELISA, WB | Humanized IgG |
| CAT | Product Name | Application | Type |
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| PABZ-173 | Human Anti-PS Recombinant Antibody (clone pYAb) | ELISA, WB | Humanized IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBZ-170 | Human Anti-PS Recombinant Antibody (clone pSTAb); Fab Fragment | ELISA, WB | Humanized Fab |
| CAT | Product Name | Application | Type |
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| PFBZ-172 | Human Anti-PS Recombinant Antibody (clone pSAb); Fab Fragment | ELISA, WB | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBZ-173 | Human Anti-PS Recombinant Antibody (clone pYAb); Fab Fragment | ELISA, WB | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBZ-170 | Human Anti-PS Recombinant Antibody (clone pSTAb); scFv Fragment | ELISA, WB | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBZ-172 | Human Anti-PS Recombinant Antibody (clone pSAb); scFv Fragment | ELISA, WB | Humanized scFv |
| CAT | Product Name | Application | Type |
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| PSBZ-173 | Human Anti-PS Recombinant Antibody (clone pYAb); scFv Fragment | ELISA, WB | Humanized scFv |
| CAT | Product Name | Application | Type |
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| TAB-1082CL | Anti-Human PS Recombinant Antibody (2E5) | ELISA |
| CAT | Product Name | Application | Type |
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| TAB-1083CL | Anti-Human PS Recombinant Antibody (2E7) | ELISA |
| CAT | Product Name | Application | Type |
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| TAB-1082CL-S(P) | Anti-Human PS Recombinant Antibody scFv Fragment (2E5) | ELISA |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1083CL-S(P) | Anti-Human PS Recombinant Antibody scFv Fragment (2E7) | ELISA |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1082CL-F(E) | Anti-Human PS Recombinant Antibody Fab Fragment (2E5) | ELISA |
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