Mouse Anti-PolyQ Tract Recombinant Antibody (clone MW1) (CAT#: PABL-308)
Recombinant Mouse Antibody (MW1) is capable of binding to PolyQ.
Specific Inquiry
Figure 1 Western blot analysis of 3B5H10 and MW1 IgG binding to huntingtin exon 1 fusion proteins with variable numbers of glutamines.
Both MW1 and 3B5H10 IgGs bound to huntingtin exon 1 proteins with normal and expanded polyQ repeats, but did not bind the TRX-tag control (top panels). Equimolar loading of huntingtin exon 1 fusion proteins was verified by blotting with the N17 antibody that recognizes the first 17 residues of huntingtin (bottom panel).
Owens, G. E., New, D. M., West Jr, A. P., & Bjorkman, P. J. (2015). Anti-PolyQ antibodies recognize a short PolyQ stretch in both normal and mutant Huntingtin Exon 1. Journal of molecular biology, 427(15), 2507-2519.
Figure 2 Dot blot analysis of 3B5H10 and MW1 IgG binding to huntingtin exon 1 fusion proteins (top panels). Equimolar loading of huntingtin proteins was verified by blotting with anti-TRX (bottom panel).
Owens, G. E., New, D. M., West Jr, A. P., & Bjorkman, P. J. (2015). Anti-PolyQ antibodies recognize a short PolyQ stretch in both normal and mutant Huntingtin Exon 1. Journal of molecular biology, 427(15), 2507-2519.
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Mouse
- Type
- Mouse IgG lambda
- Specificity
- PolyQ Tract
- Clone
- MW1
- Applications
- WB, IHC, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Polyglutamine; PolyQ
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-786 | Recombinant Anti-polyQ tract Antibody | ELISA, WB, Neut, FuncS | IgG |
| MHH-786 | Recombinant Human Anti-polyQ tract Antibody | ELISA, IF, FuncS | IgG |
| PABL-307 | Mouse Anti-PolyQ Tract Recombinant Antibody (clone 1C2) | WB, ELISA, FuncS | Mouse IgG1 |
| PABC-201 | Mouse Anti-PolyQ Tract Recombinant Antibody (clone 3B5H10) | IF, WB, FuncS, WB | Mouse IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-786-F(E) | Recombinant Anti-polyQ tract Antibody Fab Fragment | ELISA, IF, FuncS | Fab |
| MHH-786-F(E) | Recombinant Human Anti-polyQ tract Antibody Fab Fragment | WB, ELISA, FuncS | Fab |
| PFBL-307 | Mouse Anti-PolyQ Tract Recombinant Antibody (clone 1C2); Fab Fragment | WB, ELISA, FuncS | Mouse Fab |
| PFBL-308 | Mouse Anti-PolyQ Tract Recombinant Antibody (clone MW1); Fab Fragment | WB, IHC, FuncS | Mouse Fab |
| PSBC-201 | Mouse Anti-PolyQ Tract Recombinant Antibody (clone 3B5H10); scFv Fragment | WB | Mouse scFv |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-786-S(P) | Recombinant Anti-polyQ tract Antibody scFv Fragment | ELISA, WB, IP, FuncS | scFv |
| MHH-786-S(P) | Recombinant Human Anti-polyQ tract Antibody scFv Fragment | ELISA, IP, FuncS | scFv |
| PSBL-307 | Mouse Anti-PolyQ Tract Recombinant Antibody (clone 1C2); scFv Fragment | WB, ELISA, FuncS | Mouse scFv |
| PSBL-308 | Mouse Anti-PolyQ Tract Recombinant Antibody (clone MW1); scFv Fragment | WB, IHC, FuncS | Mouse scFv |
| PFBC-201 | Mouse Anti-PolyQ Tract Recombinant Antibody (clone 3B5H10); Fab Fragment | WB | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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