Mouse Anti-RSPO1 Recombinant Antibody (TAB-345CT)
CAT#: TAB-345CT
This product is a recombinant mouse antibody that recognizes RSPO1. The immunogen is recombinant human RSPO1 protein (AA 31-263). The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line THP-1
Immunofluorescent staining of human cell line THP-1 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/62923/1944_A10_1_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46154/105043_B_9_5.jpg
Normal Tissue
Figure 3 Adrenal gland
Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46154/105043_B_5_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46154/105043_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46154/105043_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46154/105043_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46154/105043_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (NTERA-2, THP-1)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000169218-RSPO1
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Specifications
- Immunogen
- Recombinant human RSPO1 protein amino acids 31-263
- Host Species
- Mouse
- Derivation
- Mouse hybridoma cell line
- Type
- Mouse IgG
- Specificity
- Human RSPO1
- Species Reactivity
- Human
- Applications
- Used for immunoassay techniques such as: Western Blot; Enzyme-linked Immunosorbent Assay; Blocking; Immunoprecipitation
- Related Disease
- Colorectal cancer, breast cancer, colon cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
REVIEWS AND Q&AS
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Reliable for Protein Detection
I've used the Mouse Anti-RSPO1 Recombinant Antibody in my lab for Western blotting to study protein interactions within the Wnt signaling pathway. It consistently produces clean, precise bands, which has significantly improved our data clarity and experimental confidence.
Essential for Colorectal Cancer Research
As someone focusing on cancer research, this antibody helps target RSPO1's role in colorectal cancer. Its high affinity makes it an indispensable tool in our experiments, particularly when analyzing protein pathways related to tumor growth and progression.
Versatile and Dependable
This antibody has proven to be versatile across different assays, maintaining consistent results whether in ELISA or immunoprecipitation formats. Its reproducibility and specificity are great assets for any research focusing on RSPO1-related pathways.
Q&As
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Which research applications is this antibody most suited for?
A: This antibody is optimized for use in several immunoassay techniques, including Western Blot, ELISA, and Immunoprecipitation. It provides robust performance in detecting RSPO1 in various samples, which is crucial for experimental accuracy.
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How does RSPO1 involvement in colorectal cancer make this antibody significant for research?
A: RSPO1 is implicated in the progression of colorectal cancer due to its regulatory role in the Wnt signaling pathway. By targeting RSPO1, researchers can investigate its contribution to tumorigenesis, potentially revealing new therapeutic targets.
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What makes this antibody suitable for long-term storage?
A: The antibody is stable for short-term storage at +4°C and long-term at -20°C with proper aliquoting to avoid repeated freeze/thaw cycles. This stability ensures the antibody retains its functionality over extended periods, making it practical for ongoing research projects.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-345CT, RRID: AB_3111916)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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COA
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-344CT | Mouse Anti-RSPO1 Recombinant Antibody (TAB-344CT) | WB, ELISA, BL, FC, IP | Mouse IgG |
| TAB-344CT-S(P) | Mouse Anti-RSPO1 Recombinant Antibody; scFv Fragment (TAB-344CT-S(P)) | WB, ELISA, BL, FC, IP | Mouse scFv |
| TAB-345CT-S(P) | Mouse Anti-RSPO1 Recombinant Antibody; scFv Fragment (TAB-345CT-S(P)) | WB, ELISA, BL, IP | Mouse scFv |
| TAB-344CT-F(E) | Mouse Anti-RSPO1 Recombinant Antibody; Fab Fragment (TAB-344CT-F(E)) | WB, ELISA, BL, FC, IP | Mouse Fab |
| TAB-345CT-F(E) | Mouse Anti-RSPO1 Recombinant Antibody; Fab Fragment (TAB-345CT-F(E)) | WB, ELISA, BL, IP | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0830MZ | Recombinant Mouse Anti-Human RSPO1 Antibody (clone 8B7) | ELISA | Mouse antibody |
| HPAB-0020-DJB | Human Anti-RSPO1 Recombinant Antibody (HPAB-0020-DJB) | FC, Block | Humanized IgG |
| HPAB-0021-DJB | Mouse Anti-RSPO1 Recombinant Antibody (HPAB-0021-DJB) | FC, Inhib, FuncS | Mouse IgG |
| ZG-074R | Mouse Anti-RSPO1 Recombinant Antibody (ZG-074R) | WB, ELISA | Mouse IgG |
| VS3-XY1391 | Mouse Anti-RSPO1 Recombinant Antibody (clone 7A6) | ELISA, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1921CQ | Recombinant Rat Anti-Rspo1 Antibody (CBL663) | Neut | IgG2b |
| NEUT-1922CQ | Recombinant Rat Anti-Rspo1 Antibody (CBL882) | Neut | IgG2 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0020-DJB-S(P) | Human Anti-RSPO1 Recombinant Antibody; scFv Fragment (HPAB-0020-DJB-S(P)) | Block | Humanized scFv |
| HPAB-0021-DJB-S(P) | Mouse Anti-RSPO1 Recombinant Antibody; scFv Fragment (HPAB-0021-DJB-S(P)) | Block | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0020-DJB-F(E) | Human Anti-RSPO1 Recombinant Antibody; Fab Fragment (HPAB-0020-DJB-F(E)) | Block | Humanized Fab |
| HPAB-0021-DJB-F(E) | Mouse Anti-RSPO1 Recombinant Antibody; Fab Fragment (HPAB-0021-DJB-F(E)) | Block | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY228 | AbPlus™ Anti-RSPO1 Magnetic Beads (VS-0424-XY228) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1974 | Anti-RSPO1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6251 | Anti-Mouse RSPO1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6250 | Anti-Human RSPO1 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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