Donkey Anti-Rabbit IgG (H&L) Antibody
CAT#: 2ND-057CQ
This product is a donkey antibody that specifically recognizes heavy (γ) chains on rabbit IgG and light chains on all rabbit immunoglobulins.
Specifications
- Immunogen
- Purified Rabbit IgG, whole molecule
- Host Species
- Donkey
- Type
- IgG
- Species Reactivity
- Rabbit
- Applications
- Used for immunoassay techniques such as: Enzyme Linked Immunosorbent Assay; Western Blot
- Conjugate
- R-PE
Product Property
- Purification
- Affinity purified
- Purity
- >95% as determined by analysis by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.2
- Preservative
- 0.05% (w/v) Sodium Azide
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Target
- Alternative Names
- Rabbit IgG (H&L); Immunoglobulin G
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Versatile Lab Staple
This Anti-Rabbit IgG (H&L) antibody is a workhorse in our laboratory. We use it for Western Blotting, ELISA, and occasionally immunocytochemistry. It consistently provides strong signals with minimal background. The fact that it recognizes both heavy and light chains ensures robust detection of our polyclonal rabbit primary antibodies. We appreciate the large pack size options which are great for high-throughput screening.
Clean Western Blots
We have tried many secondary antibodies, but this one gives some of the cleanest Western Blot results. The signal-to-noise ratio is excellent, allowing us to detect low-abundance proteins that were previously difficult to see. It is compatible with our chemiluminescent detection substrates and has shown stability even after multiple freeze-thaw cycles, although we aliquot it to be safe.
Q&As
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Does this secondary antibody detect both heavy and light chains of rabbit IgG?
A: Yes, this is an Anti-Rabbit IgG (H&L) antibody. The "H&L" designation indicates that it recognizes both the Heavy and Light chains of the rabbit immunoglobulin molecule. This ensures broad and robust detection of polyclonal rabbit primary antibodies, as it can bind to the entire antibody structure, maximizing signal intensity in your assays.
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What applications is this antibody recommended for?
A: This versatile secondary antibody is generally recommended for a wide range of standard laboratory applications, including Western Blot (WB), Enzyme-Linked Immunosorbent Assay (ELISA), and Immunohistochemistry (IHC). Its broad reactivity with rabbit IgG makes it a universal reagent for detecting rabbit primary antibodies in almost any immunoassay format you might be using in your research.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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