Recombinant Mouse Anti-Ricin Antibody (6C2) (CAT#: PABL-318)
Recombinant Mouse Antibody (6C2) is capable of binding to Ricin, expressed in Chinese Hamster Ovary cells (CHO).
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Figure 1 Validation of the RTA-6C2 Fab interface.
A, binding ability of 6C2 with RTA and RTA mutants are measured by ELISA. B, inhibition of RTA and RTA mutants enzymatic activities by 6C2. After RTA or RTA mutants were preincubated with (or without) 6C2 antibody, luciferase template mRNA was added to the rabbit reticulocyte lysates containing intact ribosomes. The relative luciferase activity, which was linear with the concentration of luciferase, was measured by GloMaxTM 96 Microplate Luminometer. C, table showing the RTA mutants.
Zhu, Y., Dai, J., Zhang, T., Li, X., Fang, P., Wang, H.,... & Guo, Y. (2013). Structural insights into the neutralization mechanism of monoclonal antibody 6C2 against ricin. Journal of Biological Chemistry, 288(35), 25165-25172.
Figure 2 SPR analysis of ribosome binding to RTA in the presence (A) and absence of the antibody 6C2 (B).
Zhu, Y., Dai, J., Zhang, T., Li, X., Fang, P., Wang, H.,... & Guo, Y. (2013). Structural insights into the neutralization mechanism of monoclonal antibody 6C2 against ricin. Journal of Biological Chemistry, 288(35), 25165-25172.
Figure 3 Pulldown analysis of ribosome binding to RTA in the presence and absence of the antibody 6C2.
Zhu, Y., Dai, J., Zhang, T., Li, X., Fang, P., Wang, H.,... & Guo, Y. (2013). Structural insights into the neutralization mechanism of monoclonal antibody 6C2 against ricin. Journal of Biological Chemistry, 288(35), 25165-25172.
Specifications
- Immunogen
- R. communis Ricin
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native R. communis Ricin
- Species Reactivity
- R. communis
- Clone
- 6C2
- Applications
- ELISA, Inhib, Pull-down assay, Neut, FuncS
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The antibody was validated for ELISA Inhibition and Pull-down assay. For details, refer to Published Data.
Target
- Alternative Names
- Ricin; Ricinus communis
- Gene ID
- 8261245
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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ScFv Antibody Products
Fab Antibody Products
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Mouse Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-318 | Recombinant Mouse Anti-Ricin Antibody scFv Fragment (6C2) | Neut, FuncS | scFv |
| PFBZ-192 | Mouse Anti-Ricin Recombinant Antibody (clone RAC18); Fab Fragment | Neut | Mouse Fab |
| HPAB-1607-FY-F(E) | Macaque Anti-Ricin Recombinant Antibody (clone REV8); scFv Fragment | WB, ELISA, IP | Macaque scFv |
| HPAB-1608-FY-F(E) | Macaque Anti-Ricin Recombinant Antibody (clone REV7); scFv Fragment | WB, ELISA, IP | Macaque scFv |
| FAMAB-0310JF-S(P) | Mouse Anti-Ricin Recombinant Antibody scFv Fragment (FAMAB-0310JF-S(P)) | WB, ELISA, FuncS | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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