Mouse Anti-Ricin Recombinant Antibody (clone RAC18) (CAT#: PABZ-192)
Recombinant Mouse Antibody (RAC18) is capable of binding to Ricinus communis ricin. RAC18's binding of this epitope is consistent with its ability to inhibit the enzymatic function of RTA in vitro.
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Figure 3 Ricin accumulates at the cell surface in the presence of neutralizing Ab.
Top. Live cells were incubated with labeled transferrin (red) and ricin (green) for 10 min in the presence of the indicated antibodies, or no antibody, and then fixed. Cells were imaged via confocal microscopy, and a central slice from a z stack is shown. In the absence of Ab (left), ricin and transferrin enter the cells by similar processes and colocalize. But in the presence of the nAb RAC18 (center), ricin is retained at the cell surface, while transferrin still enters. Non-neutralizing Ab RAC23 (right) has no effect on ricin internalization. Full z stacks of each of these photos are shown in videos S3, S4, S5, S6. These experiments have been replicated in three individual experiments, with>100 cells visualized. Bottom. The increase of cell surface ricin is confirmed, and quantified, by flow cytometry. Cells were incubated with the indicated Ab and ricin for 60 min either in the cold with sodium azide, or at 37° in tissue culture medium. The former studies passive binding at the cell surface, the latter also allows for internalization. 10,000 cells were analyzed. In either case RAC 18 results in approximately two-fold increase in cell-associated ricin.
Song, K., Mize, R. R., Marrero, L., Corti, M., Kirk, J. M., & Pincus, S. H. (2013). Antibody to ricin a chain hinders intracellular routing of toxin and protects cells even after toxin has been internalized. PLoS One, 8(4), e62417.
Specifications
- Immunogen
- Ricinus communis Ricin
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Ricinus communis ricin
- Species Reactivity
- Ricinus communis
- Clone
- RAC18
- Applications
- Neut
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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ScFv Antibody Products
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-318 | Recombinant Mouse Anti-Ricin Antibody scFv Fragment (6C2) | Neut, FuncS | scFv |
| PFBZ-192 | Mouse Anti-Ricin Recombinant Antibody (clone RAC18); Fab Fragment | Neut | Mouse Fab |
| HPAB-1607-FY-F(E) | Macaque Anti-Ricin Recombinant Antibody (clone REV8); scFv Fragment | WB, ELISA, IP | Macaque scFv |
| HPAB-1608-FY-F(E) | Macaque Anti-Ricin Recombinant Antibody (clone REV7); scFv Fragment | WB, ELISA, IP | Macaque scFv |
| FAMAB-0310JF-S(P) | Mouse Anti-Ricin Recombinant Antibody scFv Fragment (FAMAB-0310JF-S(P)) | WB, ELISA, FuncS | Mouse scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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