Hi-Affi™ Recombinant Rabbit Anti-SLC6A19 Monoclonal Antibody (DS3308AB)
CAT#: MOR-3308
Recombinant Rabbit anti-SLC6A19 monoclonal antibody for WB, IHC-P, ICC/IF, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Normal Tissue
Figure 1 High expression in small intestine
Enterocytes - Microvilli Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43207/146027_A_4_2.jpg
Normal Tissue
Figure 2 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43207/146027_A_7_4.jpg
RNA Expression
Figure 3 RNA cell line category: Group enriched (BEWO, HEL, OE19)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000174358-SLC6A19
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Specifications
- Immunogen
- Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SLC6A19 aa 350-450 (internal sequence). The exact sequence is proprietary.
- Host Species
- Rabbit
- Derivation
- Phage display library screening
- Type
- IgG
- Species Reactivity
- Mouse, Rat, Human
- Clone
- DS3308AB
- Applications
- Used for immunoassay techniques such as: Western Blot; Immunohistochemistry (Paraffin); Immunocytochemistry/Immunofluorescence
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- > 95% as determined by both RP-HPLC and SDS-PAGE analysis.
- Format
- Liquid
- Storage
- Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.
Target
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Excellent IHC And IF
This Hi-Affi™ recombinant rabbit antibody (DS3308AB) gave us beautiful staining in Immunohistochemistry and Immunofluorescence. It specifically recognizes SLC6A19 in epithelial cells. The background was minimal, and the signal was strong, which is typical for these high-affinity recombinant antibodies.
Specific Western Blot Band
We used this antibody for Western Blotting to study the neutral amino acid transporter. It produced a specific band at the expected molecular weight. The recombinant production ensures consistency, which is crucial for our comparative studies on Hartnup disorder models.
Q&As
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What makes this SLC6A19 antibody unique?
A: This is a Hi-Affi™ Recombinant Rabbit Monoclonal Antibody (clone DS3308AB). It is developed using phage display technology to ensure high affinity and specificity. It targets SLC6A19 in Human, Mouse, and Rat samples, avoiding the variability of traditional antibodies.
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Is this antibody suitable for paraffin-embedded tissue?
A: Yes, this antibody is excellent for Immunohistochemistry on paraffin-embedded sections (IHC-P). It is also validated for Western Blot (WB), Immunocytochemistry (ICC), and Immunofluorescence (IF), making it a versatile tool for studying this transporter protein.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "SLC6A19"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0812CT | Recombinant Mouse anti-Human SLC6A19 Monoclonal antibody (EML2125) | WB |
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