Recombinant Human Antibody (5F2) is capable of binding to Steroid, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-Steroid mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-Steroid proteins mAb and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Figure 1 Screening individual scFv clones and analysis of the binding affinity of the 5F2 Fab by ELISA.
(A) The culture supernatant samples of 92 individual clones producing soluble scFv–myc–pIII fusion proteins and four anti-testosterone 77 Fab producing colonies (marked with a black circle) were analysed on TES–BSA coated wells. Five scFv clones (marked with an asterisk) showing higher binding response than 77 Fab were selected for further characterization. (B) The percentage of the total binding represents the value obtained by dividing the mean binding signal, an average of four parallel measurements, at each testosterone concentration by the binding response without the competing testosterone. The IC50value of 5F2 Fab, equal to the testosterone concentration at which the binding response is 50% of the maximum, is 900 nM.
Niemi, M. H., Takkinen, K., Amundsen, L. K., Söderlund, H., Rouvinen, J., & Höyhtyä, M. (2011). The testosterone binding mechanism of an antibody derived from a naïve human scFv library. Journal of Molecular Recognition, 24(2), 209-219.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-925 | Recombinant Anti-steroid Antibody | WB, FC, FuncS | IgG |
MHH-925 | Recombinant Human Anti-steroid Antibody | WB, IP, FuncS | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-925-F(E) | Recombinant Anti-steroid Antibody Fab Fragment | ELISA, RIA, FuncS | Fab |
MHH-925-F(E) | Recombinant Human Anti-steroid Antibody Fab Fragment | WB, Dot, FuncS | Fab |
PFBL-701 | Human Anti-Steroid Recombinant Antibody (clone 5F2); Fab Fragment | ELISA | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-925-S(P) | Recombinant Anti-steroid Antibody scFv Fragment | WB, IHC, FuncS | scFv |
MHH-925-S(P) | Recombinant Human Anti-steroid Antibody scFv Fragment | FC, Neut, Biosensors, FuncS | scFv |
PSBL-701 | Human Anti-Steroid Recombinant Antibody (clone 5F2); scFv Fragment | ELISA | Human scFv |
There are currently no Customer reviews or questions for PABL-708. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.