Recombinant Mouse Anti-T. cruzi trans-sialidase Antibody (13G9)
CAT#: PABL-711
Recombinant Mouse Antibody (13G9) is capable of binding to T. cruzi trans-sialidase , expressed in Chinese Hamster Ovary cells (CHO). This antibody could effectively block its sialylation and host cell invasion capacities.
Published Data
FC
Figure 1 Flow cytometry analysis of TSs parasite expression.
TS expression on T-O, T-iTS, and T-aTS parasites was followed with mAb 13G9. Isotype control, T-O parasites, were treated with secondary antibodies only.
Pascuale, C. A., Burgos, J. M., Postan, M., Lantos, A. B., Bertelli, A., Campetella, O., & Leguizamón, M. S. (2017). Inactive trans-sialidase expression in iTS-null Trypanosoma cruzi generates virulent trypomastigotes. Frontiers in cellular and infection microbiology, 7, 430.
IF
Figure 2 Surface expression pattern of TSs.
Confocal images show a similar surface expression pattern of TSs (observed by mAb 13G9 in red).
Pascuale, C. A., Burgos, J. M., Postan, M., Lantos, A. B., Bertelli, A., Campetella, O., & Leguizamón, M. S. (2017). Inactive trans-sialidase expression in iTS-null Trypanosoma cruzi generates virulent trypomastigotes. Frontiers in cellular and infection microbiology, 7, 430.
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Specifications
- Immunogen
- T. cruzi trans-sialidase
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native T. cruzi trans-sialidase
- Species Reactivity
- T. cruzi
- Clone
- 13G9
- Applications
- FC, IF
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- This antibody has been reported for use in Flow Cytometry and Immunofluorescence.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Clone 13G9"
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- Product Name
See other products for "T. cruzi trans-sialidase"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-048 | Recombinant Anti-T. cruzi trans-sialidase VHH Single Domain Antibody | WB, IHC, FC, FuncS | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-704 | Recombinant Mouse Anti-T. cruzi trans-sialidase Antibody Fab Fragment (13G9) | ELISA, Neut, BL | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-704 | Recombinant Mouse Anti-T. cruzi trans-sialidase Antibody scFv Fragment (13G9) | ELISA, Neut, BL | scFv |
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