Anti-Human TRO Recombinant Antibody (Kiatomab) (CAT#: TAB-0623CL)
Recombinant monoclonal antibody Kiatomab is a mouse monoclonal antibody that specifically binds to human Kallikrein-2 and can be potentially used in the treatment of colon cancer.
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WB
Figure 1 Western blot and flow cytometric analysis for recognition of human and mouse KIAA1114 by Kiatomab and 3–11 mAb using 293T cells and mouse brain tissue.
Actin was used as a loading control for Western blot. In flow cytometry, KIAA1114 expression is indicated as open histogram and isotype controls are represented as shaded histogram.
Kim, S. W., Yang, H. G., Kang, M. C., Lee, S., Namkoong, H., Lee, S. W., & Sung, Y. C. (2014). KIAA1114, a full-length protein encoded by the trophinin gene, is a novel surface marker for isolating tumor-initiating cells of multiple hepatocellular carcinoma subtypes. Oncotarget, 5(5), 1226.
Figure 2 Western blot and flow cytometric analysis for recognition of human and mouse KIAA1114 by Kiatomab and 3–11 mAb using 293T cells and mouse brain tissue.
Actin was used as a loading control for Western blot. In flow cytometry, KIAA1114 expression is indicated as open histogram and isotype controls are represented as shaded histogram.
Kim, S. W., Yang, H. G., Kang, M. C., Lee, S., Namkoong, H., Lee, S. W., & Sung, Y. C. (2014). KIAA1114, a full-length protein encoded by the trophinin gene, is a novel surface marker for isolating tumor-initiating cells of multiple hepatocellular carcinoma subtypes. Oncotarget, 5(5), 1226.
FC
Figure 3 Overlapping expression between established liver TIC markers and KIAA1114.
Flow cytometric analysis of HCC cell lines stained with antibodies against CD133, EpCAM, CD90, CD24, and CD13 in conjunction with Kiatomab. The quadrant marker of each plot was set on the basis of respective isotype control staining.
Kim, S. W., Yang, H. G., Kang, M. C., Lee, S., Namkoong, H., Lee, S. W., & Sung, Y. C. (2014). KIAA1114, a full-length protein encoded by the trophinin gene, is a novel surface marker for isolating tumor-initiating cells of multiple hepatocellular carcinoma subtypes. Oncotarget, 5(5), 1226.
Specifications
- Host Species
- Mouse
- Specificity
- Human
- Clone
- Kiatomab
- Applications
- WB, FC, ELISA
- Related Disease
- Colon cancer
Applications
- Application Notes
- The TRO antibody has been reported in applications of Western Blot, Flow Cytometry, Enzyme-linked Immunosorbent Assay.
WB: Membranes were blocked 1 hour with 5% non fat dry milk in 0.1% Tween TBS (TBST) and further incubated overnight at 4°C with Kiatomab or anti-TROPHININ mAb diluted in blocking buffer.
FC: Cells were resuspended in FACS buffer (PBS/1% FBS) containing Kiatomab or puried mouse IgG2b isotype antibody and incubated on ice for 15 minutes. After being washed with FACS buffer, cells were incubated with allophycocyanin (APC)-conjugated goat anti-mouse Ig (BD Biosciences).
Target
- Alternative Names
- TRO; trophinin; MAGED3; MAGE-d3; MAGE superfamily protein; MAGE-D3 antigen; magphinin; melanoma antigen, family D, 3
- Gene ID
- 7216
- UniProt ID
- Q12816
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Mouse Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1006 | Recombinant Anti-human TRO Antibody | WB, IP, FuncS | IgG |
| MHH-1006 | Recombinant Human Anti-human TRO Antibody | WB, IF, FuncS | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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