Human Anti-VEGF Recombinant Antibody (TP-078CL) (CAT#: TP-078CL)

Figure 1 Bevacizumab (TP-078CL) in WB

Western blot analysis of TP-078CL was performed by loading Human VEGF165 Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TP-078CL and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.8 μg)

Figure 2 Bevacizumab (TP-078CL) in WB

Western blot analysis of TP-078CL was performed by loading Human VEGF121 Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TP-078CL and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.8 μg)

Figure 3 Bevacizumab (TP-078CL) in DB

Dot Blot analysis of TP-078CL was performed by coating with Human VEGF165 Protein (His Tag).

TP-078CL incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000)

Figure 4 Bevacizumab (TP-078CL) in DB

Dot Blot analysis of TP-078CL was performed by coating with Human VEGF121 Protein (His Tag).

TP-078CL incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000)

Figure 5 Bevacizumab (TP-078CL) in ELISA

ELISA analysis of TP-078CL was performed by coating with Human VEGF165 Protein (His Tag). Then blocked with BSA, and incubated with TP-078CL. The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 6 Bevacizumab (TP-078CL) in ELISA

ELISA analysis of TP-078CL was performed by coating with Human VEGF121 Protein (His Tag). Then blocked with BSA, and incubated with TP-078CL. The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.