Recombinant Anti-HIV-1 gp120 VHH Single Domain Antibody (PABX-002-VHH) (CAT#: PABX-002-VHH)

Figure 1 Dose-dependent competition of VHH A12, D7, and C8 with sCD4 for binding to recombinant envelope proteins in the ELISA.

Threefold serial dilutions of VHH were preincubated with IIIB gp120 and subsequently incubated with sCD4 precoated on microtiter plates. Envelope protein binding to sCD4 was detected as described in the text. Chemiluminescence was measured, and background subtracted luminescence readings (in relative light units [RLU) were plotted against VHH concentration.

Forsman, A., Beirnaert, E., Aasa-Chapman, M. M., Hoorelbeke, B., Hijazi, K., Koh, W., ... & Verrips, T. (2008). Llama antibody fragments with cross-subtype human immunodeficiency virus type 1 (HIV-1)-neutralizing properties and high affinity for HIV-1 gp120. Journal of virology, 82(24), 12069-12081.

Figure 2 VHH competition with each other and with sCD4 for binding to recombinant gp120 in the ELISA.

Wells of microtiter plates were coated with VHH A12, D7, and C8, as indicated above each graph. Serial dilutions of each VHH and sCD4 were preincubated with IIIB gp120 and subsequently incubated with the immobilized VHH. Envelope protein binding to VHH was detected as described in the text. Background subtracted luminescence readings were plotted against MAb concentration.

Forsman, A., Beirnaert, E., Aasa-Chapman, M. M., Hoorelbeke, B., Hijazi, K., Koh, W., ... & Verrips, T. (2008). Llama antibody fragments with cross-subtype human immunodeficiency virus type 1 (HIV-1)-neutralizing properties and high affinity for HIV-1 gp120. Journal of virology, 82(24), 12069-12081.