Bevacizumab (CAT#: TP-078CL)

A recombinant humanized monoclonal IgG1 antibody that binds to and inhibits the biologic activity of human vascular endothelial growth factor (VEGF). Bevacizumab contains human framework regions and the complementarity-determining regions of a murine antibody that binds to VEGF. Bevacizumab is produced in a Chinese Hamster Ovary mammalian cell expression system in a nutrient medium containing the antibiotic gentamicin and has a molecular weight of approximately 149 kilodaltons.


Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Tested Data
  • Datasheet
  • MSDS
  • COA
WB

Figure 1 Bevacizumab (TP-078CL) in WB

Figure 1 Bevacizumab (TP-078CL) in WB

Western blot analysis of TP-078CL was performed by loading Human VEGF165 Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TP-078CL and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.8 μg)

WB

Figure 2 Bevacizumab (TP-078CL) in WB

Figure 2 Bevacizumab (TP-078CL) in WB

Western blot analysis of TP-078CL was performed by loading Human VEGF121 Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TP-078CL and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.8 μg)

DB

Figure 3 Bevacizumab (TP-078CL) in DB

Figure 3 Bevacizumab (TP-078CL) in DB

Dot Blot analysis of TP-078CL was performed by coating with Human VEGF165 Protein (His Tag).

TP-078CL incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000)

DB

Figure 4 Bevacizumab (TP-078CL) in DB

Figure 4 Bevacizumab (TP-078CL) in DB

Dot Blot analysis of TP-078CL was performed by coating with Human VEGF121 Protein (His Tag).

TP-078CL incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000)

ELISA

Figure 5 Bevacizumab (TP-078CL) in ELISA

Figure 5 Bevacizumab (TP-078CL) in ELISA

ELISA analysis of TP-078CL was performed by coating with Human VEGF165 Protein (His Tag). Then blocked with BSA, and incubated with TP-078CL. The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

ELISA

Figure 6 Bevacizumab (TP-078CL) in ELISA

Figure 6 Bevacizumab (TP-078CL) in ELISA

ELISA analysis of TP-078CL was performed by coating with Human VEGF121 Protein (His Tag). Then blocked with BSA, and incubated with TP-078CL. The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.


Specifications

  • Type
  • Biotech
  • Trade name
  • Bevacizumab
  • UNII
  • 2S9ZZM9Q9V

Target

  • Function
  • Humanized mAb that binds all isoforms of veGFA

Related Resources

  • Biosimilar Overview
Please refer to Bevacizumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Bevacizumab.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Bevacizumab"

Anti-idiotypic Antibody

CAT Product Name Application Type
ABA-013WJ Rabbit Anti-Bevacizumab Antibody ELISA Rabbit IgG
ABA-014WJ Mouse Anti-Bevacizumab Antibody ELISA Mouse IgG1, κ
ABA-099WJ Mouse Anti-Bevacizumab Antibody (ADWJ14) Indirect ELISA, Neut Mouse IgG1, κ
ABA-100WJ Mouse Anti-Bevacizumab Antibody (ADWJ15) Indirect ELISA, ADA assay, Neut Mouse IgG1, κ
ABA-101WJ Mouse Anti-Bevacizumab Antibody (ADWJ6) PK bridge ELISA, Neut, Indirect ELISA Mouse IgG1, κ

Submit a review or a question
There are currently no Customer reviews or questions for TP-078CL. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.

For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

© 2024 Creative Biolabs.
  • 0
  • 0
Cart

    Go to compare