Recombinant Anti-EPCAM × Anti-CD3 Bispecific Antibody (Tandem Diabody)
CAT#: TAND-L045
Recombinant Anti-EPCAM × Anti-CD3 Bispecific Antibody (Tandem Diabody) is a bispecific fusion protein with four binding sites, two of which bind to antigens of one kind of cells and the other two to the other kind of cells. Tandem Diabodies do not carry Fc domains, are smaller than whole IgGs or IgG-derived BsAbs but larger than tandem scFvs. Therefore, they have a shorter serum half-life than IgGs but remain longer in the circulation compared with tandem scFvs. This BsAb is capable of targeting the tumor and recruiting T effector cells via binding to the CD3 subunit of the T cell receptor complex. It was designed for the treatment of EPCAM positive epithelial cancer.
Specifications
- Target
- EPCAM & CD3
- Type
- Tandem Diabody
- Species Reactivity
- Human
- Target 1
- EPCAM
- Host Species 1
- Human
- Target 2
- CD3
- Host Species 2
- Human
- Application
- FuncS, Promising therapeutic agent
- Related Disease
- Pancreatic cancer, Colorectal cancer
Target
- Target 1
- EPCAM
- Gene ID
- 4072
- UniProt ID
- P16422
- Alternative Names
- EPCAM; epithelial cell adhesion molecule; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1; epithelial glycoprotein 314; human epithelial glycoprotein-2; cell surface glycoprotein Trop-1; adenocarcinoma-associated an
- Target 2
- CD3
- Gene ID
- 916
- UniProt ID
- P07766
- Alternative Names
- CD3E; CD3e molecule, epsilon (CD3-TCR complex); T3E; TCRE; IMD18; T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell antigen receptor complex, epsilon subunit of T3
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for TAND-L045. Click the button above to contact us or submit your feedback about this product.
Potent Lysis Of Epithelial Cells
In our co-culture system with epithelial tumor cells and PBMCs, this Tandem Diabody induced very potent cell lysis. Its ability to recruit T cells to EpCAM positive targets is remarkable, even at relatively low effector-to-target ratios in our experiments.
Stable Tetravalent Format
We were concerned about the stability of antibody fragments, but this Tandem Diabody format is quite robust. It maintained its binding activity after freeze-thaw cycles, and the tetravalent binding provided strong signals in our FACS analysis of tumor cells.
Q&As
-
What is the target specificity of this Tandem Diabody?
A: This Tandem Diabody is specific for EpCAM and CD3. EpCAM is a widely expressed antigen on epithelial carcinomas, while CD3 is a T cell marker. This dual specificity allows the antibody to physically link T cells to EpCAM-positive tumor cells, facilitating targeted immune responses.
-
Does the tetravalent structure improve binding to tumor cells?
A: Yes, the tetravalent structure, which provides two binding sites for EpCAM, enhances the avidity of the antibody for tumor cells. This is particularly beneficial for targeting tumors with lower antigen density, ensuring stable binding and effective T cell recruitment even in challenging research models.
View the frequently asked questions answered by Creative Biolabs Support.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "EPCAM & CD3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAB-L066 | Recombinant Anti-EPCAM × Anti-CD3 Bispecific Antibody (Fab2) | FuncS, Promising therapeutic agent | Fab2 |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: ELISA, Neut, IF, IP, FC, FuncS
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, WB
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
