Chicken Anti-CERT (ab1) Polyclonal IgY
CAT#: BRD-0120MZ
This antibody is a chicken polyclonal antibody which specifically reacts with CERT (ab1).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35645/382_D7_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42608/800_A9_1_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35645/71119_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35645/71119_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35645/71119_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35645/71119_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35645/71119_A_4_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35645/71119_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (ASC diff, WM-115)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113163-COL4A3BP
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Specifications
- Immunogen
- Recombinant protein of human Alpha 3 type IV collagen binding protein isoform 2 (COL4A3BP)
- Host Species
- Chicken
- Type
- Chicken antibody
- Antibody Isotype
- IgY
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB
Target
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Reduced Background Noise
The choice of chicken as a host species provides an advantage by minimizing background signals, which enhances the clarity and reliability of our assay results in complex tissue samples.
Essential for Lipid Research
This antibody is crucial in our studies of lipid trafficking and metabolism. Its high specificity for CERT enables detailed insights into ceramide transport processes critical to cellular lipid regulation.
Aids in Understanding Disease Mechanisms
Our research into neurodegenerative and metabolic disorders greatly benefits from this antibody. It aids in linking lipid metabolism irregularities to pathological states, a critical step in developing therapeutic strategies.
Q&As
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What are the advantages of using a chicken-derived antibody for CERT research?
A: Chicken antibodies can bind to epitopes on mammalian proteins that might be less immunogenic in mammals, offering improved specificity and reducing background noise in assay results.
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How should the antibody be stored for optimal performance?
A: The antibody should be stored at -20°C to maintain stability and efficacy. Avoid repeated freeze-thaw cycles to pQAent degradation and ensure long-term usability.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "COL4A3BP"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0121MZ | CERT (ab2) Chicken Anti-CERT (ab2) Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0909MZ | Chicken Anti-COL4A3BP Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC815 | AbPlus™ Anti-COL4A3BP Magnetic Beads (VS-0724-YC815) | IP, Protein Purification |
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