PDZ domain scaffold protein anti-Human nNOS
CAT#: SPD-L171
The PDZ domain that bound nNOS was isolated from the library using Yeast Two-hybrid techonology. PDZ domains are modular protein-interaction domains that are specialized for binding to short peptide motifs at the extreme carboxy (C) termini of other proteins, athough they can also have other modes of interaction. After affinity maturation, PDZ domain variants were identified having affinities (KD=10 μM) for nNOS as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HaCaT
Immunofluorescent staining of human cell line HaCaT shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58312/1591_F5_3_selected.jpg
Normal Tissue
Figure 2 High expression in caudate nucleus
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69509/167482_B_7_7_val_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69509/167482_B_9_5.jpg
Normal Tissue
Figure 4 Kidney
Distal tubules Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69509/167482_A_7_5.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (HaCaT, RH-30, RPTEC TERT1, U-2 OS)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000089250-NOS1
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Specifications
- Scaffold Name
- PDZ domain
- Origin
- PSD-95 protein
- Core Structure
- β-sheet
- Target
- nNOS
- Species Reactivity
- Human
- Expression Host
- E. coli
- Affinity Constant
- 10 μM
- Applications
- ELISA; IHC; Microscopy; FC; WB; FuncS
Target
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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See other products for "NOS1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0790CT | Recombinant Mouse anti-Human NOS1 Monoclonal antibody (OPT-236) | IHC-P | |
| MRO-1100-CN | Recombinant Rabbit Anti-NOS1 Monoclonal Antibody (CBACN-404) | WB, IF, IHC, IP, FC | Rabbit IgG |
| MRO-2079-CN | Rabbit Anti-NOS1 Polyclonal Antibody (MRO-2079-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-CJ859 | Rabbit Anti-NOS1 Recombinant Antibody (VS3-CJ859) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| VS7-0425-WR488 | Mouse Anti-NOS1 Recombinant Antibody (clone 2E11G6) | ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2471 | Hi-Affi™ Recombinant Rabbit Anti-NOS1 Monoclonal Antibody (DS2471AB) | WB, FC, IP, IHC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC826 | CytoStream™ Rabbit Anti-NOS1 Recombinant Antibody (VS13-YC826) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4899 | Anti-NOS1 Immunohistochemistry Kit | IHC |
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