PDZ domain scaffold protein anti-Human nNOS (CAT#: SPD-L171)
The PDZ domain that bound nNOS was isolated from the library using Yeast Two-hybrid techonology. PDZ domains are modular protein-interaction domains that are specialized for binding to short peptide motifs at the extreme carboxy (C) termini of other proteins, athough they can also have other modes of interaction. After affinity maturation, PDZ domain variants were identified having affinities (KD=10 μM) for nNOS as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HaCaT
(Immunofluorescent staining of human cell line HaCaT shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58312/1591_F5_3_selected.jpg
(Immunofluorescent staining of human cell line HaCaT shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58312/1591_F5_3_selected.jpg
Figure 2 High expression in caudate nucleus
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69509/167482_B_7_7_val_selected.jpg
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69509/167482_B_7_7_val_selected.jpg
Figure 3 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69509/167482_B_9_5.jpg
(Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69509/167482_B_9_5.jpg
Figure 4 Kidney
(Distal tubules Staining: Medium Intensity: Moderate Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69509/167482_A_7_5.jpg
(Distal tubules Staining: Medium Intensity: Moderate Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69509/167482_A_7_5.jpg
Figure 5 RNA cell line category: Cell line enhanced (HaCaT, RH-30, RPTEC TERT1, U-2 OS)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000089250-NOS1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000089250-NOS1
Specifications
- Scaffold Name
- PDZ domain
- Origin
- PSD-95 protein
- Core Structure
- β-sheet
- Target
- nNOS
- Species Reactivity
- Human
- Expression Host
- E. coli
- Affinity Constant
- 10 μM
- Applications
- ELISA; IHC; Microscopy; FC; WB; FuncS
Target
- Alternative Names
- PSD-95 protein; PDZ domain; NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS
- Gene ID
- 4842
- UniProt ID
- P29475
Related Resources
AGE-RAGE Signaling Pathway in Diabetic Complications
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "NOS1"
Select a product category from the dropdown menu below to view related products.
Please select product type
IgG Antibody Products
Rabbit Monoclonal Antibody Products
Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2667z | Mouse Anti-NOS1 Recombinant Antibody (clone 20A5) | WB, ELISA, FC, ICC, IF | Mouse IgG1 |
| MOB-0790CT | Recombinant Mouse anti-Human NOS1 Monoclonal antibody (OPT-236) | IHC-P | |
| MRO-1100-CN | Recombinant Rabbit Anti-NOS1 Monoclonal Antibody (CBACN-404) | WB, IF, IHC, IP, FC | Rabbit IgG |
| MRO-2079-CN | Rabbit Anti-NOS1 Polyclonal Antibody (MRO-2079-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-CJ859 | Rabbit Anti-NOS1 Recombinant Antibody (VS3-CJ859) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for SPD-L171. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: ELISA, Neut, IF, IP, FC, FuncS
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: WB, ELISA, FuncS, IB, FC, SPR, Apop
Application: Block, IP, IF, FC
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: ELISA, PD, Activ, PK, Stim
Application: ELISA, WB
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
