CytoStream™ Rabbit Anti-NOS1 Recombinant Antibody (VS13-YC826) (CAT#: VS13-YC826)
The CytoStream™ Rabbit Anti-NOS1 Recombinant Antibody is specific for human, mouse and rat NOS1. NOS1 belongs to nitric oxide synthases, mediating neurotransmission and various signaling roles in muscle and nervous contexts, with multiple splice variants and isoforms identified. The anti-NOS1 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HaCaT
(Immunofluorescent staining of human cell line HaCaT shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58312/1591_F5_3_selected.jpg
(Immunofluorescent staining of human cell line HaCaT shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58312/1591_F5_3_selected.jpg
Figure 2 High expression in caudate nucleus
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69509/167482_B_7_7_val_selected.jpg
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69509/167482_B_7_7_val_selected.jpg
Figure 3 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69509/167482_B_9_5.jpg
(Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69509/167482_B_9_5.jpg
Figure 4 Kidney
(Distal tubules Staining: Medium Intensity: Moderate Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69509/167482_A_7_5.jpg
(Distal tubules Staining: Medium Intensity: Moderate Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69509/167482_A_7_5.jpg
Figure 5 RNA cell line category: Cell line enhanced (HaCaT, RH-30, RPTEC TERT1, U-2 OS)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000089250-NOS1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000089250-NOS1
Specifications
- Immunogen
- Recombinant human nNOS, aa 1398-1434.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat NOS1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC-P, IP, FC
- Conjugate
- NONE
- Related Disease
- Stroke and neurodegenerative diseases, neural regulation of smooth muscle
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/1000-1/2000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Nitric Oxide Synthase 1; Peptidyl-Cysteine S-Nitrosylase NOS1; Nitric Oxide Synthase 1 (Neuronal); Constitutive NOS; EC 1.14.13.39; Neuronal NOS; NOS Type I; NC-NOS;
- Gene ID
- 4842
- UniProt ID
- P29475
- Long Name
- Nitric Oxide Synthase 1
- Cellular Localization
- Cell membrane; Cell projection
Recommended Products
Secondary Antibody
Isotype Control
Related Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "NOS1"
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2667z | Mouse Anti-NOS1 Recombinant Antibody (clone 20A5) | WB, ELISA, FC, ICC, IF | Mouse IgG1 |
| MOB-0790CT | Recombinant Mouse anti-Human NOS1 Monoclonal antibody (OPT-236) | IHC-P | |
| MRO-1100-CN | Recombinant Rabbit Anti-NOS1 Monoclonal Antibody (CBACN-404) | WB, IF, IHC, IP, FC | Rabbit IgG |
| VS3-CJ859 | Rabbit Anti-NOS1 Recombinant Antibody (VS3-CJ859) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| VS7-0425-WR488 | Mouse Anti-NOS1 Recombinant Antibody (clone 2E11G6) | ICC, FC | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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