Rabbit Anti-NOS1 Recombinant Antibody (VS3-CJ859) (CAT#: VS3-CJ859)
This product is a rabbit antibody that recognizes human, mouse, and rat NOS1.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HaCaT
(Immunofluorescent staining of human cell line HaCaT shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58312/1591_F5_3_selected.jpg
(Immunofluorescent staining of human cell line HaCaT shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58312/1591_F5_3_selected.jpg
Figure 2 High expression in caudate nucleus
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69509/167482_B_7_7_val_selected.jpg
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69509/167482_B_7_7_val_selected.jpg
Figure 3 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69509/167482_B_9_5.jpg
(Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69509/167482_B_9_5.jpg
Figure 4 Kidney
(Distal tubules Staining: Medium Intensity: Moderate Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69509/167482_A_7_5.jpg
(Distal tubules Staining: Medium Intensity: Moderate Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69509/167482_A_7_5.jpg
Figure 5 RNA cell line category: Cell line enhanced (HaCaT, RH-30, RPTEC TERT1, U-2 OS)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000089250-NOS1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000089250-NOS1
Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat NOS1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC, IP, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry.
Target
- Alternative Names
- NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS
- Gene ID
- 4842
- UniProt ID
- P29475
- Sequence Similarities
- Belongs to the NOS family.
- Cellular Localization
- Cell membrane, Cell projection, Membrane, Synapse
- Post Translation Modifications
- Ubiquitinated; mediated by STUB1/ChIP in the presence of Hsp70 and Hsp40 (in vitro).
- Protein Refseq
- NP_000611.1; NP_001191142.1; NP_001191143.1
- Function
- Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
Recommended Products
Isotype Control
Secondary Antibody
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "NOS1"
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IgG Antibody Products
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Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2667z | Mouse Anti-NOS1 Recombinant Antibody (clone 20A5) | WB, ELISA, FC, ICC, IF | Mouse IgG1 |
| MOB-0790CT | Recombinant Mouse anti-Human NOS1 Monoclonal antibody (OPT-236) | IHC-P | |
| MRO-1100-CN | Recombinant Rabbit Anti-NOS1 Monoclonal Antibody (CBACN-404) | WB, IF, IHC, IP, FC | Rabbit IgG |
| MRO-2079-CN | Rabbit Anti-NOS1 Polyclonal Antibody (MRO-2079-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS7-0425-WR488 | Mouse Anti-NOS1 Recombinant Antibody (clone 2E11G6) | ICC, FC | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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