PE-A*02:01/Human PRAME (SLLQHLIGL) MHC Tetramer
CAT#: MHC-LC185
This product is a tetramer of biotinylated peptide/MHC complex with streptavidin mainly composed of PRAME-derived peptide of SLLQHLIGL sequence covering 425-433 and A*02:01 molecule. The pMHC tetramer recognizes human CD8 T cells, and can be used in the analysis of individual antigen-specific T cells.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line K-562
Immunofluorescent staining of human cell line K-562 shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68562/1854_A5_4_selected.jpg
Normal Tissue
Figure 2 Colon
Glandular cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45153/160303_A_7_3.jpg
Normal Tissue
Figure 3 Testis
Elongated or late spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Leydig cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45153/160303_A_5_6.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enriched (K-562)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000185686-PRAME/subcellular
❮
❯
Specifications
- Allele
- A*02:01
- Class
- Class I
- MHC Species
- Human
- Antigen
- PRAME
- Antigen Species
- Human
- Peptide
- SLLQHLIGL
- Range
- 425-433
- Conjugate
- PE
- Application
- FCM
Target
- Antigen Introduction
- This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants.
- Alternative Names
- PRAME; preferentially expressed antigen in melanoma; MAPE; OIP4; CT130; OIP-4
- Gene ID
- 23532
- UniProt ID
- Q05320
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Excellent for T Cell Analysis
Using this tetramer, our group has made significant progress in identifying T cell populations specific to tumor antigens. The high specificity and reliability allow us to confidently evaluate the immune response in various cancer tissue samples.
Integrates Seamlessly with Flow Cytometry
This tetramer integrates seamlessly into our flow cytometry analysis. The PE conjugate offers bright signals that are easy to detect and quantify, making our data analysis straightforward and reliable.
Key to Personalized Immunotherapy Research
The detailed T cell profiling it allows has been key to tailoring immunotherapy research to individuals' tumors in our precision medicine projects. It's a must-have for anyone engaged in personalized cancer treatment research.
Q&As
-
What is the main application of this MHC tetramer?
A: The PE-A*02:01/Human PRAME MHC Tetramer is primarily used in the analysis of antigen-specific T cells. It helps researchers identify and quantify T cell populations that recognize the PRAME peptide, providing insights into immune responses in cancer research.
-
How does this product assist in cancer research?
A: This MHC tetramer targets PRAME, a melanoma-associated antigen. By recognizing CD8 T cells specific to PRAME, it aids in evaluating immune responses and studying tumor-infiltrating lymphocytes in melanoma and other cancers where PRAME is expressed.
-
How is PRAME detected using this product?
A: Researchers use flow cytometry to identify PRAME-specific CD8 T cells. The tetramer binds specifically to these cells, allowing precise enumeration and characterization crucial for understanding tumor immunity.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "PRAME"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1701LY-S(P) | Human Anti-PRAME Recombinant Antibody; scFv Fragment (HPAB-1701LY-S(P)) | ELISA, WB, RIA | Humanized scfv |
| HPAB-1702LY-S(P) | Human Anti-PRAME Recombinant Antibody; scFv Fragment (HPAB-1702LY-S(P)) | ELISA, WB, RIA | Humanized scfv |
| HPAB-1703LY-S(P) | Human Anti-PRAME Recombinant Antibody; scFv Fragment (HPAB-1703LY-S(P)) | ELISA, WB, RIA | Humanized scfv |
| HPAB-1704LY-S(P) | Human Anti-PRAME Recombinant Antibody; scFv Fragment (HPAB-1704LY-S(P)) | ELISA, WB, RIA | Humanized scfv |
| HPAB-1705LY-S(P) | Human Anti-PRAME Recombinant Antibody; scFv Fragment (HPAB-1705LY-S(P)) | ELISA, WB, RIA | Humanized scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1701LY | Human Anti-PRAME Recombinant Antibody (HPAB-1701LY) | ELISA, WB, RIA | Humanized IgG |
| HPAB-1702LY | Human Anti-PRAME Recombinant Antibody (HPAB-1702LY) | ELISA, WB, RIA | Humanized IgG |
| HPAB-1703LY | Human Anti-PRAME Recombinant Antibody (HPAB-1703LY) | ELISA, WB, RIA | Humanized IgG |
| HPAB-1704LY | Human Anti-PRAME Recombinant Antibody (HPAB-1704LY) | ELISA, WB, RIA | Humanized IgG |
| HPAB-1705LY | Human Anti-PRAME Recombinant Antibody (HPAB-1705LY) | ELISA, WB, RIA | Humanized IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1703LY-F(E) | Human Anti-PRAME Recombinant Antibody; Fab Fragment (HPAB-1703LY-F(E)) | ELISA, WB, RIA | Humanized Fab |
| HPAB-1704LY-F(E) | Human Anti-PRAME Recombinant Antibody; Fab Fragment (HPAB-1704LY-F(E)) | ELISA, WB, RIA | Humanized Fab |
| HPAB-1705LY-F(E) | Human Anti-PRAME Recombinant Antibody; Fab Fragment (HPAB-1705LY-F(E)) | ELISA, WB, RIA | Humanized Fab |
| HPAB-1706LY-F(E) | Human Anti-PRAME Recombinant Antibody; Fab Fragment (HPAB-1706LY-F(E)) | ELISA, WB, RIA | Humanized Fab |
| HPAB-1707LY-F(E) | Human Anti-PRAME Recombinant Antibody; Fab Fragment (HPAB-1707LY-F(E)) | ELISA, WB, RIA | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5648 | Anti-PRAME Immunohistochemistry Kit | IHC |
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