PE-B*15:01/Human POU2AF1 (LPHQPLATY) MHC Tetramer (CAT#: MHC-LC4449)

Name: PE-B*15:01/Human POU2AF1 (LPHQPLATY) MHC Tetramer
SKU: MHC-LC4449
Rating: 5 (1 reviews)

This product is a tetramer of biotinylated peptide/MHC complex with streptavidin mainly composed of human POU2AF1 of peptide LPHQPLATY covering 52-60 and B*15:01 molecule. The pMHC tetramer recognizes CD8 T cells, and can be used in the analysis of individual antigen-specific T cells.

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Custom Production

MHC tetramer custom production provides a vital tool for researchers seeking to understand and manipulate T-cell immunity with exceptional precision. Please specify your requirements, including peptide sequence, MHC allele, and desired fluorophore etc. We will respond as soon as possible with a tailored solution.

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Disclaimer: Please note that the MHC reagents we offered have been produced with strict quality control (including gene sequencing, affinity purification, SDS-PAGE analysis, and verification of biotinylation), but many have not been confirmed to stain or activate specific T cells.

We require custom production

Gene Expression
Datasheet
MSDS
COA

Subcellular Location and Protein Expression

Figure 1 IHC staining of human tonsil
(Immunohistochemical staining shows high expression in tonsil)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_6_8_selected.jpg

Normal Tissue

Figure 2 Cerebral cortex
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27284_B_8_5.jpg

Figure 3 Colon
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_7_3.jpg

Figure 4 Liver
(Cholangiocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_7_4.jpg

Figure 5 Kidney
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_7_5.jpg

Figure 6 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_4_6.jpg

Figure 7 Lymph node
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_7_8.jpg

Figure 8 Tonsil
(Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_6_8.jpg

RNA Expression

Figure 9 RNA cell line category: Group enriched (Daudi, Karpas-707, U-266/70, U-266/84, U-698)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000110777-POU2AF1/subcellular

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Allele
B*15:01

Class
Class I

MHC Species
Human

Antigen
POU2AF1

Antigen Species
Human

Peptide
LPHQPLATY

Range
52-60

Conjugate
PE

Application
FCM

Target

Antigen Introduction
POU domain class 2-associating factor 1 is a protein that in humans is encoded by the POU2AF1 gene.

Alternative Names
POU2AF1; POU class 2 associating factor 1; BOB1; OBF1; OCAB; OBF-1

Gene ID
5450

UniProt ID
Q16633

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CAT	Product Name	Application	Type
MOB-1507MZ	Recombinant Mouse Anti-Human POU2AF1 Antibody (clone Xvf-BD6)	IHC-P	Mouse antibody
VS3-QX894	Mouse Anti-POU2AF1 Recombinant Antibody (clone 1170CT1.1.1)	WB	Mouse IgG1, κ
VS3-WK1013	Mouse Anti-POU2AF1 Recombinant Antibody (VS3-WK1013)	IHC	Mouse IgG2a
VS3-WK1014	Mouse Anti-POU2AF1 Recombinant Antibody (clone ABT-BOB1)	IHC-P	Mouse IgG2a, κ
VS4-CJ79	Recombinant Mouse Anti-POU2AF1 Antibody (VS4-CJ79)	ELISA, IHC	Mouse IgG2a, κ

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